Sökning: "acetate formation"

Visar resultat 1 - 5 av 63 avhandlingar innehållade orden acetate formation.

1. 1. Probing Control of Glucose Feeding in Escherichia coli Cultivations

   Författare :Mats Åkesson; Institutionen för reglerteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Automatiska system; control engineering; robotics; Automation; dissolved oxygen; acetate; glucose feeding; robotteknik; Escherichia coli; Process control; fed-batch cultivation; reglerteknik; Biotechnology; Bioteknik;

   Sammanfattning : Production of many proteins can today be made using genetically modified organisms. One of the most frequently used host organisms is the bacterium Escherichia coli. A difficulty encountered in cultivations of E. coli is the accumulation of the metabolic by-product acetate which inhibits cell growth and production of a desired protein. LÄS MER

3. 2. Coordination Chemistry of Monocarboxylate and Aminocarboxylate Complexes at the Water/Goethite Interface

   Författare :Katarina Norén; Per Persson; Staffan Sjöberg; Stephan J. Hug; Umeå universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Acetate; benzoate; adsorption; surface complex; ternary surface complexes; ATR-FTIR spectroscopy; EXAFS spectroscopy.; cyclohexanecarboxylate; sarcosine; MIDA; EDDA; EDTA; gallium III ; goethite; mineral surface; Chemistry; Kemi;

   Sammanfattning : This thesis is a summary of five papers with focus on adsorption processes of various monocarboxylates and aminocarboxylates at the water/goethite interface. Interaction of organic acids at the water/mineral interfaces are of importance in biogeochemical processes, since such processes have potential to alter mobility and bioavailability of the acids and metal ions. LÄS MER

4. 3. Metabolic engineering and cultivation strategies for recombinant production of (R)-3-hydroxybutyrate

   Författare :Mariel Perez-Zabaleta; Gen Larsson; Antonius J. A. van Maris; Diethard Mattanovich; KTH; []
   Nyckelord :Escherichia coli; R -3-hydroxybutyrate; nitrogen limitation; nitrogen depletion; lignocellulose; fed batch; acetate; β-ketothiolase; acetoacetyl-CoA reductase; Halomonas boliviensis.; Bioteknologi; Biotechnology;

   Sammanfattning : Metabolic engineering and process engineering are two powerful disciplines to design and improve microbial processes for sustainable production of an extensive number of compounds ranging from chemicals to pharmaceuticals. The aim of this thesis was to synergistically combine these two disciplines to improve the production of a model chemical called (R)-3-hydroxybutyrate (3HB), which is a medium-value product with a stereocenter and two functional groups. LÄS MER

5. 4. Growth rate control of periplasmic product retention in Escherichia coli

   Författare :Emma Bäcklund; Gen Larsson; Stefan Schmidt; Amelie Eriksson Karlström; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Escherichia coli; fed-batch; outer membrane proteins; recombinant proteins; specific growth rate; periplasmic retention; phosphotransferase system; high cell density cultivation; acetate formation; Bioengineering; Bioteknik;

   Sammanfattning : The recombinant product is secreted to the periplasm in many processes where E. coli is used as host. One drawback with secretion is the undesired leakage of the periplasmic products to the medium. The aim of this work was to find strategies to influence the periplasmic retention of recombinant products. LÄS MER

7. 5. Cobalt and Nickel Bioavailability for Biogas Formation

   Författare :Jenny Gustavsson; Bo Svensson; Anna Karlsson; Piet N.L. Lens; Linköpings universitet; []
   Nyckelord :Biogas production; bioavailability; cobalt; nickel; sequential extraction; S XANES; 454-pyrosequencing.; Biogasproduktion; biotillgänglighet; kobolt; nickel; sekventiell extraktion; S XANES; 454-pyrosekvensering;

   Sammanfattning : Supplementation of trace metals such as Co and Ni may improve anaerobic digestion of organic material for biogas formation. Which trace metals that are needed and the quantity to apply are, at least partly, related to metal speciation and bioavailability. LÄS MER