Sökning: "Bioteknologi"

Visar resultat 1 - 5 av 735 avhandlingar innehållade ordet Bioteknologi.

  1. 1. Barcoded DNA Sequencing for Parallel Protein Detection

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Mahya Dezfouli; KTH.; KTH.; [2015]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA barcoding; antibody labeling; antibody oligonucleotide bio-conjugation; DNAassisted proteomics; immuno-sequencing I-Seq ; droplet-based system; large-scale data analysis; Biotechnology; Bioteknologi;

    Sammanfattning : The work presented in this thesis describes methodologies developed for integration and accurate interpretation of barcoded DNA, to empower large-scale-omics analysis. The objectives mainly aim at enabling multiplexed proteomic measurements in high-throughput format through DNA barcoding and massive parallel sequencing. LÄS MER

  2. 2. Rational and Combinatorial Engineering of Affinity Proteins Towards Therapeutical Applications

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Johan Seijsing; KTH.; [2014]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; neonatal Fc receptor; FcRn; affibody molecule; immunoglobulin G IgG ; serum albumin SA ; albumin binding domain ABD ; half-life extension; blocking; autoimmune disease; pH-dependence; GAD67; GAD-1; gene therapy; Parkinson’s disease; zinc finger based artificial transcription factor; Biotechnology; Bioteknologi;

    Sammanfattning : Protein engineering has had an immense impact on the development of biological drugs, including replacement therapies with engineered versions of insulin or factor VIII to treat diabetes or bleeding disorders, and monoclonal antibodies to treat cancer and various other malignancies. Now, with the next generation of treatment modalities coming up, including monoclonal reagents based on alternative scaffolds, gene and cell therapies, the importance of protein engineering to tailor-make these treatments is likely to increase further. LÄS MER

  3. 3. Discovery and investigation of glycoside hydrolase family 5 enzymes with potential use in biomass conversion

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Yang Wang; KTH.; [2015]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; plant cell wall; biomass; hemicellulose; mannans; glycoside hydrolase; subfamily classification; Arabidopsis; mannanase; transglycosylase; enzymatic characterization.; Biotechnology; Bioteknologi;

    Sammanfattning : Glycoside hydrolases (GHs) cleave glycosidic bonds in glycoconjugates, oligosaccharides and polysaccharides such as cellulose and various hemicelluloses. Mannan is a major group of hemicelluloses. LÄS MER

  4. 4. Live Single Cell Imaging and Analysis Using Microfluidic Devices

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Mohammad Ali Khorshidi; KTH.; [2013]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Single cell analysis; time-lapse fluorescence imaging; automated image analysis; microwell; droplet microfluidics; NK cells; single cell tracking; migration behavior analysis; cell-cell interaction; optical microscopy; image analysis; image processing; microfluidics; immune cells; tracking; counting; morphology analysis;

    Sammanfattning : Today many cell biological techniques study large cell populations where an average estimate of individual cells’ behavior is observed. On the other hand, single cell analysis is required for studying functional heterogeneities between cells within populations. LÄS MER

  5. 5. Design and Evaluation of Radiolabeled Affibody Tracers for Imaging of HER2-expressing Tumors

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Helena Wållberg; KTH.; [2011]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Affibody molecule; radionuclide molecular imaging; HER2; radiotracer; SPECT; PET; biodistribution; protein engineering; radiolabeling;

    Sammanfattning : The growing understanding of tumor biology and the identification of tumor specificgenetic and molecular alterations, such as the overexpression of human epidermal growthfactor receptor 2 (HER2), opens up for personalization of patient management using targeted therapies. However, this puts stringent demands on the diagnostic tools usedto identify patients that are likely to respond to a particular treatment. LÄS MER