Sökning: "Bioteknologi"

Visar resultat 1 - 5 av 792 avhandlingar innehållade ordet Bioteknologi.

  1. 1. Barcoded DNA Sequencing for Parallel Protein Detection

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Mahya Dezfouli; KTH.; KTH.; [2015]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA barcoding; antibody labeling; antibody oligonucleotide bio-conjugation; DNAassisted proteomics; immuno-sequencing I-Seq ; droplet-based system; large-scale data analysis; Biotechnology; Bioteknologi;

    Sammanfattning : The work presented in this thesis describes methodologies developed for integration and accurate interpretation of barcoded DNA, to empower large-scale-omics analysis. The objectives mainly aim at enabling multiplexed proteomic measurements in high-throughput format through DNA barcoding and massive parallel sequencing. LÄS MER

  2. 2. Rational and Combinatorial Engineering of Affinity Proteins Towards Therapeutical Applications

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Johan Seijsing; KTH.; [2014]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; neonatal Fc receptor; FcRn; affibody molecule; immunoglobulin G IgG ; serum albumin SA ; albumin binding domain ABD ; half-life extension; blocking; autoimmune disease; pH-dependence; GAD67; GAD-1; gene therapy; Parkinson’s disease; zinc finger based artificial transcription factor; Biotechnology; Bioteknologi;

    Sammanfattning : Protein engineering has had an immense impact on the development of biological drugs, including replacement therapies with engineered versions of insulin or factor VIII to treat diabetes or bleeding disorders, and monoclonal antibodies to treat cancer and various other malignancies. Now, with the next generation of treatment modalities coming up, including monoclonal reagents based on alternative scaffolds, gene and cell therapies, the importance of protein engineering to tailor-make these treatments is likely to increase further. LÄS MER

  3. 3. Bioparticle Manipulation using Acoustophoresis and Inertial Microfluidics

    Detta är en avhandling från Stockholm, Sweden : Kungliga Tekniska högskolan

    Författare :Muhammad Asim Faridi; KTH.; [2017]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biotechnology; Bioteknologi;

    Sammanfattning : Despite the many promising advances made in microfluidics, sample preparation remains the single largest challenge and bottleneck in the field of miniaturised diagnostics. This thesis is focused on the development of sample preparation methods using active and passive particle manipulation techniques for point of care diagnostic applications. LÄS MER

  4. 4. Microfluidic based isolation of circulating tumor cells from whole blood for cancer diagnostics

    Detta är en avhandling från stockholm : KTH Royal Institute of Technology

    Författare :Harisha Ramachandraiah; KTH.; [2017]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biotechnology; Bioteknologi;

    Sammanfattning : Detection of circulating tumor cells (CTC) in peripheral blood is indicative of early recognition of tumor progression and such an important biomarker for early diagnosis, staging, monitoring and prognosis of cancer. However, CTC are found in very low concentrations and reliable isolation of these rare cells is challenging. LÄS MER

  5. 5. Discovery and investigation of glycoside hydrolase family 5 enzymes with potential use in biomass conversion

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Yang Wang; KTH.; [2015]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; plant cell wall; biomass; hemicellulose; mannans; glycoside hydrolase; subfamily classification; Arabidopsis; mannanase; transglycosylase; enzymatic characterization.; Biotechnology; Bioteknologi;

    Sammanfattning : Glycoside hydrolases (GHs) cleave glycosidic bonds in glycoconjugates, oligosaccharides and polysaccharides such as cellulose and various hemicelluloses. Mannan is a major group of hemicelluloses. LÄS MER