Sökning: "Dissociation constant"

Visar resultat 26 - 30 av 57 avhandlingar innehållade orden Dissociation constant.

1. 26. Tunnels and Grooves : Structure-Function Studies in Two Disparate Enzymes

   Författare :Daniel Ericsson; Sherry Mowbray; Guy Dodson; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mycobacterium tuberculosis; ribonucleotide reductase; peptide inhibitors; fluorescence polarization; lipase; interfacial activation; hydrolase; X-ray structure; substrate specificity; Structural biology; Strukturbiologi;

   Sammanfattning : This thesis describes structural and binding studies in enzymes from two different  organisms: ribonucleotide reductase from Mycobacterium tuberculosis (RNR) and lipase A from Candida antarctica (CalA).RNR is viable as a target for new drugs against the causative agent of tuberculosis. LÄS MER

3. 27. Biochemical Studies of Semenogelins with Emphasis on Zinc-Binding Properties

   Författare :Magnus Jonsson; Malmö Klinisk kemi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; sexualitet; sexuality; Obstetrik; gynekologi; andrologi; reproduction; andrology; gynaecology; Obstetrics; Klinisk kemi; Clinical chemistry; gel-forming proteins; surface plasmon resonance; prostate-specific antigen; protein C inhibitor; seminal plasma; zinc; semen; semenogelin; reproduktion;

   Sammanfattning : Semenogelins I and II (SgI and SgII) are homologous and rapidly evolving proteins that have a repetitive primary structure and are synthesized mainly in the seminal vesicles. Upon ejaculation, a gelatinous coagulum is formed when the semenogelin-rich secretion from the seminal vesicles is mixed with the prostate secretion, which contains high concentrations of zinc(II) and proteases. LÄS MER

4. 28. Development of new NMR techniques and the structure of the N-terminal domain of Escherichia coli DnaB helicas

   Författare :Johan Weigelt; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :NMR spectroscopy; DnaB; helicase;

   Sammanfattning : New techniques applicable in biomolecular nuclear magnetic resonance (NMR) spectroscopy were developed. The three-dimensional structure of the N-terminal domain of Escherichia coli DnaB helicase was determined at atomic resolution using heteronuclear multidimensional NMR techniques. LÄS MER

5. 29. Quantitation, Purification and Reconstitution of the Red Blood Cell Glucose Transporter GLUT1

   Författare :Shusheng Zuo; Bengt Mannervik; Bengt Persson; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Cholesterol; Cytochalasin B; Glucose; GLUT1; Hummel and Dreyer analysis; Immobilization; PEG 5000 -DSPE; Biomembrane; Proteoliposome; Quantitative frontal affinity chromatography; Red blood cell streptavidin-biotin immobilization; Sulfhydryl affinity chromatography; The modified micro-Bradford CaPE assay; MALDI-ToF-MS; Biokemi; Biochemistry; Biokemi;

   Sammanfattning : The human glucose transporter GLUT1 facilitates glucose to be accumulated on the other side of the cell membrane. The functional state of GLUT1 is uncertain due to diversity of the reports. In this thesis, the activity of red blood cell GLUT1 was extensively studied to further characterize this protein. LÄS MER

7. 30. Studies of ligand- and temperature-gated ion channels using microfluidic methods

   Författare :Maria Millingen; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; solution exchange; desensitization; temperature-gated; ion channel; temperature control; ligand-gated; TRPV1; GABAA receptor; microfluidics;

   Sammanfattning : This thesis describes the studies of the GABAA receptor, a ligand-gated ion channel, and the ligand- and temperature-gated ion channel TRPV1. The desensitization behavior of both of these proteins has been studied for different conditions, using a microfluidic device for superfusion. LÄS MER