Avancerad sökning

Hittade 2 avhandlingar som matchar ovanstående sökkriterier.

1. 1. High cell density perfusion process development for antibody producing Chinese Hamster Ovary cells

   Författare :Ye Zhang; Vincent Bulone; Veronique Chotteau; Carl-Fredrik Mandenius; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; WAVE Bioreactor; Cellbag; ATF; TFF; hollow fiber; CSPR; CellTank; SUB; single-use-bioreactor; disposable; perfusion rate; Biotechnology; Bioteknologi;

   Sammanfattning : Perfusion operation mode is currently under fast expansion in mammalian cell based manufacturing of biopharmaceuticals, not only for labile drug protein but also for stable proteins such as monoclonal antibodies (mAbs). Perfusion mode can advantageously offer a stable cell environment, long-term production with high productivity and consistent product quality. LÄS MER

3. 2. Tools for the development of intensified perfusion processes for mammalian cell culture

   Författare :Hubert Schwarz; Véronique Chotteau; Mark Smales; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Recombinant protein biomanufacturing; Perfusion process; Integrated continuous bioprocessing; Monoclonal antibody; Erythropoietin; Chinese Hamster Ovary cells; Human Embryonic Kidney cells; Perfusion medium optimization; Cell specific perfusion rate; Raman spectroscopy; N-glycosylation; Microbioreactors; Design of Experiments; Biotechnology; Bioteknologi;

   Sammanfattning : Recombinant protein therapeutics have become an indispensable part of modern medicine to treat a wide variety of diseases. Their manufacture is mostly based on mammalian cells with fed-batch production bioreactors and downstream batch purification unit operations. LÄS MER