Sökning: "protein throughput"

Visar resultat 1 - 5 av 259 avhandlingar innehållade orden protein throughput.

1. 1. Proteome wide protein production

   Författare :Hanna Tegel; Sophia Hober; Diethard Mattanovich; KTH; []
   Nyckelord :protein production; Escherichia coli; transcription; promoter; translation; rare codon; high-throughput; screening;

   Sammanfattning : Over a decade after the completion of the human genome, researchers around the world are still wondering what information is hidden in the genome. Although the sequences of all human genes are known, it is still almost impossible to determine much more than the primary protein structure from the coding sequence of a gene. LÄS MER

3. 2. Protein-protein interactions in model systems : design, control of catalytic activity and biosensor applications

   Författare :Johan Rydberg; Lars Baltzer; Joel Schneider; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chemistry; Protein interactions; design; catalysis; biosensors; hybride materials; nanoparticles; Kemi; Chemistry; Kemi;

   Sammanfattning : This thesis describes the design of polypeptides, unordered in the monomeric state but capable of folding into helix-loop-helix motifs and dimerise to form four-helix bundles. The goal of the design was to encode them with the capacity to form dimers highly selectively and the ability to carry out molecular functions in the folded state but not in the unordered state, and thus to establish a molecular link between recognition and function. LÄS MER

4. 3. High-throughput protein analysis using mass spectrometry-based methods

   Författare :Tove Boström; Sophia Hober; Thomas Laurell; Ole N. Jensen; KTH; []
   Nyckelord :Proteomics; mass spectrometry; affinity proteomics; immunoenrichment; immunoprecipitation; IMAC; screening; protein production; protein purification; ISET; quantification; SILAC; stable isotope standard; antibody validation; Bioteknologi; Biotechnology;

   Sammanfattning : In the field of proteomics, proteins are analyzed and quantified in high numbers. Protein analysis is of great importance and can for example generate information regarding protein function and involvement in disease. Different strategies for protein analysis and quan- tification have emerged, suitable for different applications. LÄS MER

5. 4. Characterization of Antigenic Properties and High Throughput Protein Purification

   Författare :Johanna Steen; Sophia Hober; Mats Ohlin; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; high throughput; protein purification; sample preparation; antigen solubility; immunogenicity; epitope mapping; antigen design; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : To understand the cellular processes, knowledge of the localization and function of proteins are essential. There are several high throughput ventures examining the human proteome. However, there are some bottlenecks in these ventures. For example the production and expression of soluble proteins for analysis. LÄS MER

7. 5. Platforms and strategies harnessing signaling pathways of multifactorial diseases by multispecific antibodies

   Författare :Aman Mebrahtu; Johan Rockberg; Fredrik Y. Frejd; Janine Schuurman; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibodies; Bispecific antibodies; Multispecific affinity proteins; Protein engineering; Protein expression; Cancer; Immunotherapy; Autoimmunity; Systemic Lupus Erythematosus; Affibody molecules; AffiMab; Antikroppar; Bispecifika antikroppar; Multispecifika affinitets protein; Protein engineering; Protein uttryck; Cancer; Immunoterapai; Autoimmunitet; Systemic Lupus Erythematosus; Affibody molekyler; AffiMab; Biotechnology; Bioteknologi;

   Sammanfattning : Abstract [ENG]Proteins govern a multitude of biological functions vital to sustain life. The ability to withstand diseases development and harboring a defense against foreign pathogens is attributed to the wonders of the immune system and the proteins and cells its comprised of. LÄS MER