Sökning: "Lars Baltzer"

Visar resultat 1 - 5 av 11 avhandlingar innehållade orden Lars Baltzer.

1. 1. Exploring molecular interactions between polypeptide conjugates and protein targets : Manipulating affinity by chemical modifications

   Författare :Aleksandra Balliu; Lars Baltzer; Oliver Seitz; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; polypeptide conjugates; protein targets; molecular recognition; affinity enhancement; conjugation;

   Sammanfattning : In this thesis molecular interactions between polypeptide conjugates and protein targets were investigated. Polypeptides were derivatized with small organic molecules, peptides and oligonucleotides. New strategies were developed with the aim to increase affinities for proteins of biological interest. LÄS MER

3. 2. Targeting RNA by the Antisense Approach and a Close Look at RNA Cleavage Reaction

   Författare :Jharna Barman; Lars Baltzer; Roger Strömberg; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; mRNA targeting; antisense oligonucleotides; target affinity; RNase H; Michaelis-Menten kinetics; exo-nuclease stability; NMR; pH titration; alkaline hydrolysis; Organisk kemi;

   Sammanfattning : This thesis summarizes the results of studies on two aspects of nucleic acids. Chemically modified antisense oligonucleotides (AONs) have been evaluated with regards to their suitability for mRNA targeting in an antisense approach (Paper I – III). LÄS MER

4. 3. Catalysis and Site-Specific Modification of Glutathione Transferases Enabled by Rational Design

   Författare :Sofia Håkansson Hederos; Lars Baltzer; Kerstin Broo; Linköpings universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Catalysis; Glutathione Transferases; Rational design; Protein design; Site-specific modification; thiolester hydrolysis; Enzyme engineering; Enzymteknik;

   Sammanfattning : This thesis describes the rational design of a novel enzyme, a thiolester hydrolase, derived from human glutathione transferase (GST) A1-1 by the introduction of a single histidine residue. The first section of the thesis describes the design and the determination of the reaction mechanism. LÄS MER

5. 4. Chemical Engineering of Small Affinity Proteins

   Författare :Joel Lindgren; Amelie Eriksson Karlström; Lars Baltzer; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Affibody molecules; albumin binding domain; ligation; protein synthesis; solid phase peptide synthesis; Bioteknologi; Biotechnology;

   Sammanfattning : Small robust affinity proteins have shown great potential for use in therapy, in vivo diagnostics, and various biotechnological applications. However, the affinity proteins often need to be modified or functionalized to be successful in many of these applications. LÄS MER

7. 5. Expanding the Amino Acid Alphabet by Design : Enhanced and Controlled Catalytic Activity in Folded Polypeptide Catalysts

   Författare :N. Johan V. Lindgren; Lars Baltzer; Harri Lönnberg; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Chemistry; Kemi; Organic Chemistry; organisk kemi;

   Sammanfattning : This thesis addresses structure and reactivity of polypeptide catalysts in reactions that mimic the hydrolysis of RNA and DNA. A designed helix-loop-helix motif was used as a scaffold where the amino acid residues were systematically varied. LÄS MER