Sökning: "Roman Zubarev"
Hittade 5 avhandlingar innehållade orden Roman Zubarev.
1. Mass spectrometry of biopolymers based on ion-induced desorption
Sammanfattning : Various aspects of biopolymer molecular characterization by their molecular masses havebeen studied, with emphasis on ion-induced desorption and time-of-flight mass Spectrometry(TOF MS). Limitations of the average-isotopic mass concept, as well as the utility ofaccurate monoisotopic mass measurements for deriving amino-acid and elementalcompositions, are discussed. LÄS MER
2. Secondary and Higher Order Structural Characterization of Peptides and Proteins by Mass Spectrometry
Sammanfattning : The work in this thesis has demonstrated the advantages and limitations of using MS based technologies in protein and peptide structural studies. Tandem MS, specifically electron capture dissociation (ECD) have shown the ability to provide structural insights in molecules containing the slightest of all modifications (D-AA substitution). LÄS MER
3. Multiplexed absolute quantification of blood plasma proteins using targeted proteomics
Sammanfattning : Proteins are the molecular building blocks of all living organisms. They are the functioning actors in metabolism and communication, and they give specific architecture and purpose to all tissues. Their abundance is dynamic and can be interpreted and associated to different physiological states or diseases. LÄS MER
4. Characterization of Polypeptides by Tandem Mass Spectrometry Using Complementary Fragmentation Techniques
Sammanfattning : In the growing field of proteomics identification of proteins by tandem mass spectrometry (MS/MS) is performed by matching experimental mass spectra against calculated spectra of all possible peptides in a protein database. One problem with this approach is the false-positive identifications. LÄS MER
5. New Proteomics Methods and Fundamental Aspects of Peptide Fragmentation
Sammanfattning : The combination of collision-activated dissociation, (CAD) and electron capture dissociation, (ECD) yielded a 125% increase in protein identification. The S-score was developed for measuring the information content in MS/MS spectra. This measure made it possible to single out good quality spectra that were not identified by a search engine. LÄS MER