Sökning: "Matrix-Assisted Laser Desorption Ionization Mass Spectrometry"

Visar resultat 16 - 20 av 50 avhandlingar innehållade orden Matrix-Assisted Laser Desorption Ionization Mass Spectrometry.

1. 16. High-throughput screening of solid-phase extraction materials using mass spectrometry

   Författare :Kishore Jagadeesan; Avdelningen för Biomedicinsk teknik; []
   Nyckelord :mass spectromery; protein separation; sample preparation; Molecularly imprinted polymer; Data analysis; Data visualization; Miniaturisation; Proteomics;

   Sammanfattning : In biomarker analysis, sample preparation plays a crucial step in order to extract, isolate and concentrate the analytes of interest. Among the various sample preparation techniques, solid phase extraction (SPE) is one of the most common and popular, due to the high enrichment factor, good recovery, low consumption of organic solvents and the possibility to automate (off- or on-line) the whole process. LÄS MER

3. 17. New Tools for Analysis of Wood and Pulp Components - Application of MALDI-TOF Mass Spectroscopy and Capillary Zone Electrophoresis

   Författare :Anna Jacobs; KTH; []
   Nyckelord :Capillary zone electrophoresis; carbohydrate composition; degree of acetylation; degree of polymerization; enzymatic hydrolysis; hardwood; hemicellulose; hexenuronic acid; lignin; MALDI-TOF mass spectroscopy; molar mass; molar mass distribution; olig;

   Sammanfattning : In the present thesis, capillary zone electrophoresis (CZE),matrix-assisted laser desorption/ionization time-of-flight massspectroscopy (MALDI-MS) and size-exclusion chromatography incombination with off-line MALDI-MS (SEC/MALDI-MS) have beenapplied successfully to the analysis of wood and pulpcomponents.The carbohydrate compositions of pulp and holocellulosesamples, as well as of isolated hemicellulose preparations,were determined employing enzymatic hydrolysis and subsequentanalysis of the saccharides released by CZE. LÄS MER

4. 18. Improved techniques for CE-MALDI-MS off-line coupling and MALDI-MS analysis of primarily hydrophobic proteins and peptides

   Författare :Johan Jacksén; Åsa Emmer; Anna Karin/Minna Borg Karlsson/Hakkarainen; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon microcanal; Matrix; Protein cleavage digestion.; Kapillärelektrofores; Hydrofoba peptider; Matris-assisterad laserdesorptions-joniserings-masspektrometri; Strukturbelagd provplatta; Off-line interface; Mikrokanal i kisel; Matris; Proteinklyvning -spjälkning.; Analytical chemistry; Analytisk kemi;

   Sammanfattning : Due to the hydrophobic nature of integral membrane proteins (IMP) they give rise to several difficulties concerning handling and analysis, which is not the case for the most water soluble proteins. New analysis methods are needed, where the insolubility problems of the hydrophobic proteins due to aggregation and adhesion are tackled. LÄS MER

5. 19. Improved techniques for CE and MALDI-MS including microfluidic hyphenations foranalysis of biomolecules

   Författare :Johan Jacksén; Åsa Emmer; Tomas Bergman; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides proteins; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon micro canal; Matrix; Peptide-fluorescein isothiocyanate adducts; Bovine serum albumin; DHAP; Hyphenations; Hydrophobicity; Single wood fiber analysis; Pre-concentration; Concentration platform; Analytical chemistry; Analytisk kemi;

   Sammanfattning : In this thesis, improved techniques for biomolecule analysis using capillary electrophoresis (CE) and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and hyphenations between those have been presented.A pre-concentration method which is possible to apply in both techniques, has also been investigated. LÄS MER

7. 20. Mass spectrometry in peptide and tumor marker analysis

   Författare :Ann-Charlotte Bergman; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Integration of preparative protein and peptide separations with analytical techniques, in particular mass spectrometry (MS), is important. This thesis describes preparation via capillary electrophoresis and two-dimensional (2-D) gel electrophoresis for identification and structure determination of polypeptides. LÄS MER