Sökning: "Gram-positive bacteria. protein engineering"

Hittade 5 avhandlingar innehållade orden Gram-positive bacteria. protein engineering.

1. 1. Engineering of staphylococcal surfaces for biotechnological applications

   Författare :Henrik Wernérus; KTH; []
   Nyckelord :affibody; albumin binding protein; Bacterial surface display; cell immobilisation; bioremediation; combinatorial protein engineering; flow cytometry; Gram-positive; metal-binding; staphylococcal protein A; Staphylococcus carnosus; Staphylococcus xylo;

   Sammanfattning : The engineering of bacterial surfaces has in recent yearsattracted a lot of attention with applications in manydifferent areas of bioscience. Here we describe the use of twodifferent surface display systems for the gram-positivebacteria Staphylococcus carnosus and Staphylococcus xylosus invarious biotechnological applications. LÄS MER

3. 2. Staphylococcal surface display for protein engineering and characterization

   Författare :John Löfblom; Stefan Ståhl; Henrik Wernérus; K. Dane Wittrup; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; affibody; combinatorial library; epitope mapping; Gram-positive bacteria; protein engineering; staphylococcal surface display; Molecular biology; Molekylärbiologi;

   Sammanfattning : Even though our understanding of mechanisms such as protein folding and molecular recognition is relatively poor, antibodies and alternative affinity proteins with entirely novel functions are today generated in a routine manner. The reason for this success is an engineering approach generally known as directed evolution. LÄS MER

4. 3. Staphylococcal surface display in directed evolution

   Författare :Nina Kronqvist; Stefan Ståhl; Mats A. A. Persson; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; combinatorial library; directed evoluation; Gram-positive bacteria. protein engineering; Bioengineering; Bioteknik;

   Sammanfattning : Engineered affinity proteins have together with naturally derived antibodies becomeindispensable tools in many areas of life-science and with the increasing number ofapplications, the need for high-throughput methods for generation of such different affinityproteins is evident. Today, combinatorial protein engineering is the most successful strategy toisolate novel non-immunoglobulin affinity proteins. LÄS MER

5. 4. Bacterial display systems for engineering of affinity proteins

   Författare :Filippa Fleetwood; John Löfblom; Harald Kolmar; KTH; []
   Nyckelord :Combinatorial protein engineering; staphylococcal display; Affibody; biparatopic; VEGFR2; nanobody; E. coli display; autotransporter; Bioteknologi; Biotechnology;

   Sammanfattning : Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. LÄS MER

7. 5. Poly (3-hydroxybutyrate) from a moderate halophile: production and modification for new applications

   Författare :Marlene Munoz; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biopolymers; polyhydroxyalkanoates; polyhydroxybutyrate; moderate halophile; Halomonas boliviensis; fermentation; recovery; thermally induced phase separation; scaffolds; covalent modification; hydrophobic ion pairing; polyethylenemine; lysozyme.;

   Sammanfattning : Polyhydroxybutyrate (PHB) is a homopolymer belonging to the family of biopolyesters, polyhydroxyalkanoates (PHA) produced by different bacteria and archaea as intracellular granules often under conditions of metabolic stress due to a limited supply of essential nutrients. The properties of the PHAs are similar to those of polyethylene and polypropylene and can hence replace these fossil based polymers in a large variety of applications. LÄS MER