Sökning: "metal-binding"

Visar resultat 1 - 5 av 37 avhandlingar innehållade ordet metal-binding.

1. 1. Structural studies of metal-binding proteins revealing dynamic behaviour

   Författare :Maria Håkansson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Kemi; Chemistry; Molekylär biofysik; Molecular biophysics; zinc; magnesium; superantigen; calbindin D9k; X-ray crystallography; staphylococcal enterotoxin; structure; Chemical technology and engineering; Kemiteknik och kemisk teknologi;

   Sammanfattning : The Mg2+ and Mn2+ structures of calbindin D9k have been determined and they reveal a conformational change compared to the structures of apo and (Ca2+)2-calbindin D9k. These findings are unique, since normally calcium and not magnesium is known to induce conformational changes of EF-hand proteins. LÄS MER

3. 2. Engineering of staphylococcal surfaces for biotechnological applications

   Författare :Henrik Wernérus; KTH; []
   Nyckelord :affibody; albumin binding protein; Bacterial surface display; cell immobilisation; bioremediation; combinatorial protein engineering; flow cytometry; Gram-positive; metal-binding; staphylococcal protein A; Staphylococcus carnosus; Staphylococcus xylo;

   Sammanfattning : The engineering of bacterial surfaces has in recent yearsattracted a lot of attention with applications in manydifferent areas of bioscience. Here we describe the use of twodifferent surface display systems for the gram-positivebacteria Staphylococcus carnosus and Staphylococcus xylosus invarious biotechnological applications. LÄS MER

4. 3. Structural and physicochemical studies on metal and porphyrin binding by the Bacillus subtilis ferrochelatase

   Författare :Mattias Hansson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; metal specificity; metal binding; porphyrin metallation; time resolved crystallography; fluorescence quenching; X-ray structure; inclusion bodies; Bacillus subtilis; heme biosynthesis; ferrochelatase; hemH;

   Sammanfattning : The enzyme ferrochelatase (EC 4.99.1.1) is the terminal enzyme in the biosynthetic pathway of heme b and catalyzes the insertion of Fe(II) into protoporphyrin IX. LÄS MER

5. 4. Crystallographic studies on porphyrin metallation by ferrochelatase

   Författare :David Lecerof; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; thermal and mechanical properties; Condensed matter:stucture; fasjämvikt; kristallografi; egenskaper termiska och mekaniska ; Kondenserade materiens egenskaper:struktur; crystallography; phase equilibria; Magnesium; pi-helix; Zinc; ferrochelatase; Metal binding; porphyrin metallation;

   Sammanfattning : Ferrochelatase catalyses the terminal step in heme biosynthesis by inserting a ferrous ion into protoporphyrin IX. The reaction mechanism has in this thesis been studied by mainly crystallographic methods. LÄS MER

7. 5. Zinc in folding and misfolding of SOD1 : Implications for ALS

   Författare :Lina Leinartaité; Mikael Oliveberg; Bengt-Harald Jonsson; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein stability; protein misfolding; local unfolding; metal binding; energy landscape; protein disease; amyotrophic lateral sclerosis; Biochemistry; biokemi;

   Sammanfattning : Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease causing degeneration of upper and lower motor neurons. Most ALS cases are sporadic; only 6% are associated with mutations in Cu, Zn superoxide dismutase (SOD1). LÄS MER