Sökning: "autotransporter"

Visar resultat 1 - 5 av 9 avhandlingar innehållade ordet autotransporter.

  1. 1. Surface expression using the AIDA autotransporter : Towards live vaccines and whole-cell biocatalysis

    Författare :Martin Gustavsson; Gen Larsson; Per Berglund; Andres Veide; Jan-Willem de Gier; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; AIDA-autotransporter; Escherichia coli; live vaccines; surface expression;

    Sammanfattning : The area of surface expression has gathered a lot of interest from research groups all over the world and much work is performed in the area. Autotransporters have been used for surface expression in Gram-negative bacteria. LÄS MER

  3. 2. Combinatorial Protein Engineering Of Affibody Molecules Using E. Coli Display And Rational Design Of Affibody-Based Tracers For Medical Imaging

    Författare :Ken G. Andersson; John Löfblom; Stefan Ståhl; Harald Kolmar; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; directed evolution; microbial display; E. coli; Affibody molecule; autotransporter; medical imaging; HER receptor family; Sortase A; Bioteknologi; Biotechnology;

    Sammanfattning : Directed evolution is today an established strategy for generation of new affinity proteins. This thesis describes the development of a cell-display method using Escherichia coli for directed evolution of Affibody molecules. LÄS MER

  4. 3. Bacterial display systems for engineering of affinity proteins

    Författare :Filippa Fleetwood; John Löfblom; Harald Kolmar; KTH; []
    Nyckelord :Combinatorial protein engineering; staphylococcal display; Affibody; biparatopic; VEGFR2; nanobody; E. coli display; autotransporter; Bioteknologi; Biotechnology;

    Sammanfattning : Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. LÄS MER

  5. 4. Impact of glucose uptake rate on recombinant protein production in Escherichia coli

    Författare :Emma Bäcklund; Gen Larsson; Olle Holst; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; AIDA-autotransporter; Escherichia coli; fed-batch; glucose uptake rate; integral membrane proteins; outer membrane proteins; periplasmic retention; phosphotransferase system; recombinant proteins; specific growth rate; surface expression; Biology; Biologi;

    Sammanfattning : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. LÄS MER

  7. 5. Improved detection and performance of surface expression from the AIDA-I autotransporter

    Författare :Johan Jarmander; Gen Larsson; Ulrika Rova; Ines Ezcurra; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; surface expression; autotransport; AIDA-I; Escherichia coli; proteolysis; detection tag;

    Sammanfattning : Surface expression of recombinant proteins has attracted a lot of attention due to its potential in applications such as enzyme production, vaccine delivery and bioremediation. Autotransporters have been used for surface expression of a variety of proteins, but the expression systems reported in literature have typically been inflexible and incapable of detecting proteolysis, thereby limiting surface expression yield. LÄS MER