Sökning: "protein stabilisation"

Visar resultat 1 - 5 av 19 avhandlingar innehållade orden protein stabilisation.

1. 1. Metal Affinity Peptides in Biotechnological Applications As tools in protein purification, protein stabilisation, metal quantification and metal tolerance of bacteria and plants

   Författare :Malin Mejàre; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cadmium; metal quantification; fusion tag; metal affinity tag; phage display; peptide library; metal tolerance; metal resistance; metal accumulation; bioremediation; transgenic plant; tobacco; potato; protein purification; immobilised metal affinity chromatography; metal affinity precipitation; metal detection; protein stabilisation; Bioteknik; Biotechnology; Biokemi; Metabolism; Biochemistry; metal ion;

   Sammanfattning : Metal affinity tags have been fused to proteins and demonstrated to function as tools in protein purification, protein stabilisation, quantitative metal analysis and metal tolerance of bacteria and plants. In biotechnological applications proteins frequently have to function in nonnatural environments with harsh conditions which impose requirements for high stability on the proteins. LÄS MER

3. 2. Modelling the Protein-DNA Interface

   Författare :Malin Lüking; Shina C. Lynn Kamerlin; Ehmke Pohl; Uppsala universitet; []
   Nyckelord :Protein-DNA interactions; Molecular Dynamics; Coarse-grained simulations; gene regulation; Biokemi; Biochemistry;

   Sammanfattning : Protein-DNA interactions are crucial to life. Several millions of DNA base pair steps are organ- ised, read and protected by proteins in every cell. Protein-DNA interactions must be specific, controllable and reasonably fast. Understanding how these features coexist is one of the great challenges for biochemists and molecular biologists. LÄS MER

4. 3. Radical generation and stabilisation in ribonucleotide reductase R2

   Författare :Martin Högbom; Lawrence Que; Stockholms universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Biokemi; Biochemistry; biokemi;

   Sammanfattning : Diiron carboxylate proteins contain a cofactor that consists of two iron atoms coordinated by carboxylate and histidine ligands. These proteins perform a multitude of chemical reactions in the cell that generally involve activation of molecular oxygen at the diiron site. LÄS MER

5. 4. POLYPEPTIDES - STRUCTURES AND INTERACTIONS IN MONOLAYERS AND AQUEOUS SOLUTIONS

   Författare :Helen Sjögren; Fysikalisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Physical chemistry; Fysikalisk kemi; tyrosine ; co-poly-L lysine; phenylalanine ; co-poly-L- lysine; poly-L-glutamate; poly-L-lysine; poly-L-valine; poly-L-isoleucine; poly-gamma-methyl-L-glutamate; poly-L-leucine; monolayer; Langmuir-Blodgett film; CD spectroscopy; random coil; alpha-helix; surfactant stabilisation; polypeptide; polyamino acid;

   Sammanfattning : The aim of this thesis work is to understand and be able to control polypeptide conformation and interactions at interfaces and in solution by changing the solution conditions. For this purpose simple model polypeptides, so-called polyamino acids, have been investigated at the air-liquid interface, as well as in aqueous solutions. LÄS MER

7. 5. A molecular chaperone that governs membrane contact sites and lipid metabolism

   Författare :Sergi Tosal Castaño; Sabrina Büttner; W. Mike Henne; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Yeast; nucleus-vacuole junctions; proteostasis; unfolded protein response; fatty acid elongation; molekylär biovetenskap; Molecular Bioscience;

   Sammanfattning : Transmembrane proteins represent almost one third of the total cellular proteome, and the majority of them requires translocation into the endoplasmic reticulum (ER) to be folded, sorted and transported to their final subcellular destination. High translational rates during exponential cell growth result in a massive influx of immature proteins into the ER, requiring rapid and accurate folding. LÄS MER