Sökning: "metal affinity precipitation"

Visar resultat 1 - 5 av 9 avhandlingar innehållade orden metal affinity precipitation.

  1. 1. Metal Affinity Peptides in Biotechnological Applications As tools in protein purification, protein stabilisation, metal quantification and metal tolerance of bacteria and plants

    Författare :Malin Mejàre; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cadmium; metal quantification; fusion tag; metal affinity tag; phage display; peptide library; metal tolerance; metal resistance; metal accumulation; bioremediation; transgenic plant; tobacco; potato; protein purification; immobilised metal affinity chromatography; metal affinity precipitation; metal detection; protein stabilisation; Bioteknik; Biotechnology; Biokemi; Metabolism; Biochemistry; metal ion;

    Sammanfattning : Metal affinity tags have been fused to proteins and demonstrated to function as tools in protein purification, protein stabilisation, quantitative metal analysis and metal tolerance of bacteria and plants. In biotechnological applications proteins frequently have to function in nonnatural environments with harsh conditions which impose requirements for high stability on the proteins. LÄS MER

  3. 2. Specific Precipitation of Biomolecules Using Synthetic Polymers

    Författare :Per-Olof Wahlund; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Polymerteknik; RNA; plasmid DNA; DNA; polyelectrolyte complexes; synthetic polymers; precipitation; affinity precipotation; Bioteknik; Polymer technology; biopolymers;

    Sammanfattning : Purification is a very important stage in biotechnological processes. The traditionally used precipitation techniques are generally nonspecific and thus one or more chromatographic steps are normally required. There is thus a need for new approaches to improve existing techniques. LÄS MER

  4. 3. Genetically Engineered Plants: Improved stress tolerance, growth and facilitated protein purification

    Författare :Gösta Lilius; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glycine betaine; gene; freezing; flooding; drought; dicoteledones; desiccation; compatible solutes; cold; choline dehydrogenase; chill; CDH; betaine; betA; alkaloids anoxia; affinity purification; affinity tags; handles; hemoglobin; hypoxia; low temperatu; Biotechnology; Bioteknik;

    Sammanfattning : Heterologous genes were expressed in plants in which the overall goal was to increase the value of the final crop. Various aspects were explored and evaluated, such as improving the stress tolerance, enhancing the availability of oxygen in growing cells and producing rare proteins with increased degrees of purity. LÄS MER

  5. 4. Colloidal carrier substances : Properties and impact on trace metal distribution in natural waters

    Författare :Anna Ledin; Linköpings universitet; []
    Nyckelord :SAMHÄLLSVETENSKAP; SOCIAL SCIENCES; INTERDISCIPLINARY RESEARCH AREAS; TVÄRVETENSKAPLIGA FORSKNINGSOMRÅDEN;

    Sammanfattning : Colloidal (0,45 J.Im) are important constituents in the aquatic environment, due to their impact on the mobility and bioavailability of associated components (e.g. metals). LÄS MER

  7. 5. Alternative Adsorption Materials for Treatment of Contaminated Waters

    Författare :Yuliya Kalmykova; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Adsorption; Sphagnum peat; Heavy metals; Fiber ash;

    Sammanfattning : The thesis presents a study of adsorbent materials for treatment of contaminated waters and remediation of contaminated soils. Natural and waste product materials showed high adsorption capacity for metals and organic pollutants and proved to be a good alternative to commercial adsorbents. LÄS MER