Sökning: "Total internal reflection fluorescence"

Visar resultat 6 - 10 av 24 avhandlingar innehållade orden Total internal reflection fluorescence.

1. 6. Lipid Vesicle Fusion: Investigation, Generation and Manipulation of Cell-Membrane Mimics

   Författare :Lisa Simonsson; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; diffusion; spreading lipid bilayers; cell mimic; micromanipulation; lipid bilayer; microfluidics; site-specificity; DNA; membrane-receptor interactions; total internal reflection fluorescence microscopy; model system; self-assembly; TIRF microscopy; FRET; exocytosis; vesicles; fluorescence resonance energy transfer; biomembranes; amperometry; membrane fusion;

   Sammanfattning : Membrane fusion is essential for nerve-cell communication, for protein transport between cell organelles and the cell-membrane and for enabling the merger between virus and host membranes during virus infection. In this work, cell-membrane mimics were constructed and evaluated as models for studies of the membrane-fusion process. LÄS MER

3. 7. Unraveling the molecular mechanisms of herpes simplex virus attachment and release using cell membrane mimics

   Författare :Nadia Peerboom; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; total internal reflection fluorescence microscopy; glycosaminoglycans; atomic force microscopy; binding kinetics; single particle tracking; herpes simplex virus;

   Sammanfattning : The herpes simplex virus is a widespread human pathogen, most commonly known for causing cold sores. Its infection cycle is initiated with the formation of multiple bonds between viral glycoproteins and cellular glycosaminoglycans, which are long polysaccharide chains found close to the cell surface. LÄS MER

4. 8. Tethered Lipid Vesicles as Nanoscale Sensor Elements

   Författare :Seyed Tabaei; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; aquaglyceroporin; lipid vesicles; total internal reflection fluorescence TIRF microscopy; cell membrane; aquaporin; pore formation; surface plasmon resonance SPR ; phospholipase A2; antiviral peptide; lipid bilayer; biosensing; microfluidics;

   Sammanfattning : The primary aim of this thesis work has been to explore and advance the potential of surface tethered lipid vesicles as a platform for studying in particular the following three biomembrane-related processes: trans-membrane transport, membrane-peptide interactions and the activity of membrane active enzymes. First, a method based on surface plasmon resonance (SPR) spectroscopy was developed for studying passive diffusion processes as well as the membrane-protein mediated transport of small non electrolyte molecules across lipid bilayers. LÄS MER

5. 9. Raman and Fluorescence Spectroscopy of Single Molecules and Metal Particles

   Författare :Jerk Bjerneld; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; laser-induced; single-molecule spectroscopy; surface-enhanced Raman scattering; nanoparticles;

   Sammanfattning : Technological and conceptual advances have led to the realization of single-molecule spectroscopy. This permit detailed investigations of heterogeneous systems in fields ranging from condensed matter physics to biochemistry. It was recently discovered that surface-enhanced Raman scattering (SERS) can be used to study single molecules. LÄS MER

7. 10. Supported Lipid Membranes and Their Use for the Characterization of Biological Nanoparticles

   Författare :Silver Jõemetsa; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; fluorescence microscopy; supported lipid bilayer; lipophilic dyes; size determination; diffusion; microfluidics;

   Sammanfattning : Biological nanoparticles (BNPs) are nano-sized lipid vesicles of biological origin, which are involved in multiple biological processes. BNP characterization techniques are critical for improving the understanding of how these particles contribute to cellular communication, viral infections and drug-delivery applications. LÄS MER