Sökning: "total internal reflection fluorescence TIRF microscopy"

Visar resultat 1 - 5 av 9 avhandlingar innehållade orden total internal reflection fluorescence TIRF microscopy.

  1. 1. Characterization of artificial and biological lipid vesicles using TIRF and SPR

    Författare :Thomas Olsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; membrane permeability; chromaffin vesicle; fluorescence; total internal reflection TIRF microscopy; surface plasmon resonance SPR ; vesicle; osmotic shock; cell membrane; liposome; nanoparticle tracking analysis NTA ; large dense core vesicles LDCVs ;

    Sammanfattning : Synthetic lipid vesicles serve as important mimics of cells and the natural membranes that they are enclosed by. As such they are frequently used as simplified models of the highly complex cell membrane to aid in-depth physicochemical and biological characterization of this essential biological structure. LÄS MER

  3. 2. DNA-Controlled Lipid-Membrane Fusion

    Författare :Lisa Simonsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; exocytosis; vesicles; DNA; FRET; fluorescence resonance energy transfer; model system; total internal reflection fluorescence microscopy; TIRF microscopy; self-assembly; site-specificity; diffusion; biomembranes; lipid bilayer; membrane fusion;

    Sammanfattning : Membrane fusion is essential for nerve-cell communication, for protein transport between cell organelles and the cell-membrane and for enabling the merger between virus and host membranes during virus infection. We have demonstrated that short DNA oligonucleotides, membrane-attached via CH in an orientation that mimics the overall zipperlike architecture of fusion-inducing proteins, induce fusion of both suspended vesicles and vesicles site-specifically tethered to SLBs. LÄS MER

  4. 3. Lipid Vesicle Fusion: Investigation, Generation and Manipulation of Cell-Membrane Mimics

    Författare :Lisa Simonsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; diffusion; spreading lipid bilayers; cell mimic; micromanipulation; lipid bilayer; microfluidics; site-specificity; DNA; membrane-receptor interactions; total internal reflection fluorescence microscopy; model system; self-assembly; TIRF microscopy; FRET; exocytosis; vesicles; fluorescence resonance energy transfer; biomembranes; amperometry; membrane fusion;

    Sammanfattning : Membrane fusion is essential for nerve-cell communication, for protein transport between cell organelles and the cell-membrane and for enabling the merger between virus and host membranes during virus infection. In this work, cell-membrane mimics were constructed and evaluated as models for studies of the membrane-fusion process. LÄS MER

  5. 4. Caveolae associated proteins and how they effect caveolae dynamics

    Författare :Björn Morén; Richard Lundmark; Volker Haucke; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Caveolae; caveolin; EHD2; cavin; microdomain; microscopy; TIRF; AFM; medicinsk biokemi; Medical Biochemistry;

    Sammanfattning : Caveolae are a type of invaginated membrane domain that has been shown to be involved in several disease states, including lipodystrophy, muscular dystrophies and cancer. Several of these diseases are caused by the lack of caveolae or caveolae-related signaling deficiencies in the tissues in which the caveolar domain are abundant such as lung, adipose, muscle and their related endothelial cells. LÄS MER

  7. 5. Supported Lipid Membranes and Their Use for the Characterization of Biological Nanoparticles

    Författare :Silver Jõemetsa; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; fluorescence microscopy; supported lipid bilayer; lipophilic dyes; size determination; diffusion; microfluidics;

    Sammanfattning : Biological nanoparticles (BNPs) are nano-sized lipid vesicles of biological origin, which are involved in multiple biological processes. BNP characterization techniques are critical for improving the understanding of how these particles contribute to cellular communication, viral infections and drug-delivery applications. LÄS MER