Sökning: "MALDI TOF MS MS"

Visar resultat 16 - 20 av 44 avhandlingar innehållade orden MALDI TOF MS MS.

  1. 16. galactmannan degradation by fungi and gut bacteria : structural enzymology and fine -tuned substrate specifcity

    Författare :Sumitha Krishnaswamyreddy; Biokemi och Strukturbiologi; []
    Nyckelord :fine-tuned substrate specificity; β-mannanase; β-mannosidases; synergy; α-galactosidases; mannan converison in gut; transglycosylation; polysaccharide utilisation locus; phylogenetic analysis; MALDI-TOF MS;

    Sammanfattning : AbstractThe degradation of plant based β-mannan polysaccharides represents one of the many challenges efficiently tackled by microorganisms living in different habitats. In this thesis, glycoside hydrolases (GHs) involved in mannan degradation from two different organisms, Aspergillus nidulans (paper I and II) and Bacteroides ovatus (paper III and IV) were studied. LÄS MER

  3. 17. Transglycosylation catalyzed by β-mannanases : Molecular aspects relevant for enzymatic synthesis

    Författare :Johan Morrill; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; β-mannanase; glycoside hydrolase; enzymology; kinetics; MALDI-TOF MS; HPLC; transglycosylation; alcoholysis; enzyme engineering; protein structure; enzymatic synthesis;

    Sammanfattning : β-Mannanases are a major type of enzyme involved in the hydrolysis and modification of β-mannans, a hemicellulose present in high amounts in e.g. softwoods such as spruce. The β-mannanases studied in this thesis belong to glycoside hydrolase (GH) families GH5 and GH26. LÄS MER

  4. 18. Enzymatic conversion of β-mannans: Analysing, evaluating and modifying transglycosylation properties of glycoside hydrolases

    Författare :Mathias Wiemann; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; beta-mannanase; alpha-galactosidase; enzyme engineering; transglycosylation; MALDI-TOF MS; HPLC;

    Sammanfattning : Retaining glycoside hydrolases are enzymes that catalyse the breakdown down of glycans through hydrolysis. Due to the double-replacement mechanism of the retaining glycoside hydrolases (GHs), which form an intermediate with part of the glycan covalently attached to the enzyme, some GHs are able to catalyse synthesis reactions called transglycosylation. LÄS MER

  5. 19. Improved techniques for CE and MALDI-MS including microfluidic hyphenations foranalysis of biomolecules

    Författare :Johan Jacksén; Åsa Emmer; Tomas Bergman; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides proteins; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon micro canal; Matrix; Peptide-fluorescein isothiocyanate adducts; Bovine serum albumin; DHAP; Hyphenations; Hydrophobicity; Single wood fiber analysis; Pre-concentration; Concentration platform; Analytical chemistry; Analytisk kemi;

    Sammanfattning : In this thesis, improved techniques for biomolecule analysis using capillary electrophoresis (CE) and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) and hyphenations between those have been presented.A pre-concentration method which is possible to apply in both techniques, has also been investigated. LÄS MER

  7. 20. Clinical and molecular studies of β-haemolytic streptococci

    Författare :Kristina Trell; Infektionsmedicin; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Beta-hemolytic streptococci; Streptococcus dysgalactiae; MALDI-TOF MS analysis; Streptococcus equi; Streptococcus pyogenes; endometritis; Erysipelas;

    Sammanfattning : Beta-haemolytic streptococci (BHS) are important causes of human infections and they have traditionally been grouped according to Lancefield antigens. The spectrum of infections caused by BHS includes pharyngitis, skin infections, bacteraemia/sepsis, endocarditis, septic arthritis and meningitis. LÄS MER