Sökning: "In vitro assays"

Visar resultat 1 - 5 av 321 avhandlingar innehållade orden In vitro assays.

1. 1. Regulation of Ovarian Aromatase: Studies by Aromatase Assays in vitro and in vivo

   Författare :Dmitrijus Kirilovas; Mats Brännström; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Obstetrics and gynaecology; aromatase; human; granulosa cells; 11C-vorozole; active site; allosteric configuration; in vitro; in vivo; PET; androgens; rat; estrous cycle.; Obstetrik och kvinnosjukdomar; Obstetrics and women s diseases; Obstetrik och kvinnosjukdomar; Obstetrics and Gynaecology; obstetrik och gynekologi;

   Sammanfattning : An in vitro method was developed for measuring aromatase, based on binding of competitive aromatase inhibitor [11C]vorozole to the active site of the enzyme. [11C]Vorozole displayed high, specific binding in vitro to human placenta and human granulosa cells (GC), both fresh and frozen/thawed cells, provided correct procedures were used. LÄS MER

3. 2. In silico tools in risk assessment : of industrial chemicals in general and non-dioxin-like PCBs in particular

   Författare :Mia Stenberg; Patrik Andersson; Miriam Jacobs; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chemometrics; industrial chemicals; in silico tools; molecular descriptors; non-dioxin-like PCBs; partial least-squares PLS ; principal component analysis PCA ; quantitative structure-activity relationship QSAR ; REACH; risk assessment RA ; statistical molecular design; structure-activity relationship SAR ;

   Sammanfattning : Industrial chemicals in European Union produced or imported in volumes above 1 tonne annually, necessitate a registration within REACH. A common problem, concerning these chemicals, is deficient information and lack of data for assessing the hazards posed to human health and the environment. LÄS MER

4. 3. Acetylcholine in Spinal Pain Modulation : An in vivo Study in the Rat

   Författare :Klas Abelson; Urban Höglund; Ernst Brodin; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Laboratory animals; Pain; Nociception; Antinociception; Acetylcholine; Muscarinic; Nicotinic; Spinal cord; Microdialysis; Försöksdjursvetenskap; Laboratory animal science; Försöksdjursvetenskap;

   Sammanfattning : The spinal cord is an important component in the processing and modulation of painful stimuli. Nerve signals from the periphery are relayed and further conducted to the brain (nociception) in the spinal cord, and the most essential modulation of painful information (antinociception) occurs here. LÄS MER

5. 4. Chemosensitivity in Breast Cancer

   Författare :Kenneth Villman; Carl Blomqvist; Ulf Tidefelt; Nils Wilking; Uppsala universitet; []
   Nyckelord :Oncology; breast cancer; chemotherapy; anthracycline; predictive; topoisomerase; TOP2A; HER2; cyclin A; in vitro; Onkologi;

   Sammanfattning : Breast cancer mortality in Sweden is now in decline, thanks to early detection and the wide use of adjuvant endocrine therapy and chemotherapy. While hormone receptor status is predictive of response to endocrine treatment, there is no clinically useful predictive marker of a patient’s response to chemotherapy. LÄS MER

7. 5. Gliadin-Blocking Peptides In vitro assessment of their potential to alleviate celiac disease development

   Författare :Karolina Hoffmann; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; high-gliadin affinity blocking peptides; Caco-2 cells; in vitro digestion; tissue transglutaminase; celiac disease; gliadin; α-amylase trypsin inhibitors;

   Sammanfattning : This thesis work aimed to investigate the potential of synthetic peptides as agents to block wheat prolamins (gliadins) that trigger the development of symptoms in celiac disease. The first step of this investigation included selection and synthesis of peptides with high affinity to gliadins and assessment of the potential of these so-called blocking peptides to limit gliadin reactivity in vitro. LÄS MER