Sökning: "Diagnosis of Viral RNA"

Visar resultat 1 - 5 av 22 avhandlingar innehållade orden Diagnosis of Viral RNA.

1. 1. Detection and Quantification of Variable Viral RNA by Real-Time PCR Assays

   Författare :Shaman Muradrasoli; Jonas Blomberg; Göran Friman; Maria Brytting; Uppsala universitet; []
   Nyckelord :Virology; Diagnosis of Viral RNA; QPCR; RNA virus; broadly targeted PCR; probe design strategy; Infectious diseases; Medicin; Medicine;

   Sammanfattning : As the area of nucleic acid based technologies develops, so will our understanding of how structural variations in DNA and RNA pathogens are associated with disease. The overall goal of this thesis is the development of broadly targeted measurement techniques for variable viral RNA by Real-Time PCR (here referred to as quantitative reverse transcriptase PCR, QRT-PCR). LÄS MER

3. 2. Molecular Diagnosis of Common Viral Infectious Diseases Based on Real-Time PCR

   Författare :Nahla Mohamed; Jonas Blomberg; Jan Fohlman; Elgh Fredrik; Uppsala universitet; []
   Nyckelord :Molecular biology; Microbiology; Virology; rTth DNA polymerase; UNG; QPCR; RNA virus; broadly targeted PCR; Molekylärbiologi;

   Sammanfattning : Molecular biology has become an integral part of the diagnosis of infectious diseases. Recently, quantitative real-time PCR (QPCR) methods (often in the form of so-called TaqMan® systems) have been developed for the diagnosis of a wide range of infectious diseases; these techniques found valuable clinical application in the diagnosis and evaluation of progress and therapeutic success of viral diseases. LÄS MER

4. 3. Padlock Probe-Based Nucleic Acid Amplification Tests : Point-of-care Diagnostics of Infectious Diseases

   Författare :Sibel Ciftci; Mats Nilsson; Jonas Klingström; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Nucleic Acid Amplification; Isothermal Amplification Methods; Padlock Probes; Rolling Circle Amplification; Molecular Diagnostics; Infectious Disease Diagnostics; Virus; Point-of-Care; Biochemistry; biokemi;

   Sammanfattning : Recent advancements in molecular biology and biotechnology have pushed the field of molecular diagnostics much further to benefit the society towards smart access for rapid and simplified health care. In this context, point-of-care (PoC) technologies that bring the inventions in diagnostics closer to bedside settings draw attention. LÄS MER

5. 4. Direct Demonstration of Influenza Virus and Mycoplasma Pneumoniae in the Respiratory Tract

   Författare :Anna Nilsson; Enheten för infektionssjukdomar; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; polymeras chain reaction; influenza virus; Mycoplasma pneumoniae; diagnosis; respiratory tract infection; sample site; serial samples;

   Sammanfattning : Diagnostic tests based on nucleic acid amplification have become available for Mycoplasma pneumoniae (MP) and influenza viruses. Here, an MP PCR test detected 100% of infections during the first three symptomatic weeks, whereas classical serology detected 21% week 1, 56% week 2, and 100% week 3. LÄS MER

7. 5. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies

   Författare :Amal Elfaitouri; Jonas Blomberg; Helene Norder; Uppsala universitet; []
   Nyckelord :Microbiology; Real-time PCR; broadly targeted PCR; degenerated PCR; rTth DNA polymerase; thermolabile UNG; QPIA; IgM; Mikrobiologi;

   Sammanfattning : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. LÄS MER