Sökning: "rTth DNA polymerase"

Hittade 5 avhandlingar innehållade orden rTth DNA polymerase.

1. 1. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies

   Författare :Amal Elfaitouri; Jonas Blomberg; Helene Norder; Uppsala universitet; []
   Nyckelord :Microbiology; Real-time PCR; broadly targeted PCR; degenerated PCR; rTth DNA polymerase; thermolabile UNG; QPIA; IgM; Mikrobiologi;

   Sammanfattning : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. LÄS MER

3. 2. Molecular Diagnosis of Common Viral Infectious Diseases Based on Real-Time PCR

   Författare :Nahla Mohamed; Jonas Blomberg; Jan Fohlman; Elgh Fredrik; Uppsala universitet; []
   Nyckelord :Molecular biology; Microbiology; Virology; rTth DNA polymerase; UNG; QPCR; RNA virus; broadly targeted PCR; Molekylärbiologi;

   Sammanfattning : Molecular biology has become an integral part of the diagnosis of infectious diseases. Recently, quantitative real-time PCR (QPCR) methods (often in the form of so-called TaqMan® systems) have been developed for the diagnosis of a wide range of infectious diseases; these techniques found valuable clinical application in the diagnosis and evaluation of progress and therapeutic success of viral diseases. LÄS MER

4. 3. Detection and Characterisation of Salmonella in Animal Feed Samples by PCR-Based Methods

   Författare :Charlotta Löfström; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Food and drink technology; Livsmedelsteknik; Biotechnology; Bioteknik; Biokemisk teknik; Biochemical technology; Analytisk kemi; Analytical chemistry; mykologi; virologi; bakteriologi; Mikrobiologi; validation; Microbiology; bacteriology; virology; mycology; Salmonella; ribotyping; detection; animal feed; randomly amplified polymorphic DNA; pulsed-field gel electrophorsis; pre-PCR processing; PCR; polymerase chain reaction; genotyping;

   Sammanfattning : Animal feed is a recognised source of Salmonella enterica for farm livestock and may also indirectly cause infection in people consuming foods of animal origin. It is therefore important to have rapid, reproducible and specific methods for the detection of Salmonella in feed, and for the characterisation of strains for further epidemiological investigations or to trace the source of contamination in a production facility. LÄS MER

5. 4. Diagnostic PCR for the Detection of Yersinia enterocolitica and Salmonella in the Food-Chain: Reliability of PCR Performance

   Författare :Rickard Knutsson; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; real-time PCR; YPCE medium; Enrichment PCR; DNA polymerases; PCR inhibitors; pre-PCR processing; Salmonella; Diagnostic PCR; Yersinia enterocolitica; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

   Sammanfattning : This thesis deals with the methodological advances of diagnostic PCR including reliability of PCR and pre-PCR processing of food and feed samples. Diagnostic PCR has been greatly improved by the introduction of the second generation of PCR, so-called real-time PCR. LÄS MER

7. 5. Optimisation of diagnostic PCR a study of PCR inhibitors in blood and sample pretreatment

   Författare :Waleed Abu Al-Soud; Avdelningen för medicinsk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; mycology; virology; bacteriology; Microbiology; AmpliTaq Gold; Amplification facilitators; Betaine; Blood culture medium; Blood; BSA; cheese; faeces; gp32; Haemoglobin; Ions; Immunoglobulin G; Mikrobiologi; bakteriologi; virologi; mykologi;

   Sammanfattning : PCR is widely employed as a rapid, sensitive and specific molecular diagnostic technique in clinical diagnosis, environmental investigations and for monitoring biotechnical processes. However, the full potential of diagnostic PCR is limited, in part, by the presence of PCR-inhibitory substances in biological samples. LÄS MER