Sökning: "Bacterial Display"

Visar resultat 6 - 10 av 69 avhandlingar innehållade orden Bacterial Display.

1. 6. Engineering Antibody Specificity Using Combinatorial Antibody Libraries

   Författare :Jenny Boström; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; antibody Antigen Interactions; Dual Specific Antibodies; Herceptin; Human Epidermal Growth Factor Receptor 2; Thermodynamic Analysis; therapeutic Antibody Engineering; Synthetic Phagedisplayed Antibody Libraries; Vascular Endothelial Growth Factor; Bacterial Display; trastuzumab;

   Sammanfattning : The research presented in this thesis investigates antibody specificity, antibody-antigen and protein-protein interactions using combinatorial antibody libraries, phage-display and bacterial display technology. One goal was to develop a method to generate antibodies that bind two protein antigens with high affinity using a single-antigen binding site, and to investigate the molecular and thermodynamic requirements for antibody multispecificity. LÄS MER

3. 7. Engineering strategies for ABD-derived affinity proteins for therapeutic and diagnostic applications

   Författare :Mikael Åstrand; Sophia Hober; John Löfblom; Larry Gold; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ABD; ADAPT; Protein G; ERBB2; ERBB3; Directed evolution; phage display; staphylococcal display; bispecific; Bioteknologi; Biotechnology;

   Sammanfattning : Small stable protein domains are attractive scaffolds for engineering affinity proteins due to their high tolerance to mutagenesis without loosing structural integrity. The albuminbinding domain is a 5 kDa three-helix bundle derived from the bacterial receptor Protein G with low-nanomolar affinity to albumin. LÄS MER

4. 8. Interaction engineered three-helix bundle domains for protein recovery and detection

   Författare :Tove Alm; Sophia Hober; Rainer Hahn; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ABD; Zbasic; albumin; protein engineering; phage display; staphylococcal display; inclusion bodies; refolding; proteomics; orthogonal affinity purification; Bioengineering; Bioteknik; Immunology engineering; Immunteknik;

   Sammanfattning : HTML clipboard The great advances in DNA technology, e.g. sequencing and recombinant DNA techniques, have given us the genetic information and the tools needed to effectively produce recombinant proteins. Recombinant proteins are valuable means in biotechnological applications and are also emerging as alternatives in therapeutic applications. LÄS MER

5. 9. The biology of filamentous phage infection - implications for display technology

   Författare :Fredrik Karlsson; Institutionen för immunteknologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biotechnology; Bioteknik; infection mechanism; selective infection; TolA; Escherichia coli; pIII; Filamentous phage; Phage display technology;

   Sammanfattning : Phage display technology is a biotechnological tool that can be used to obtain molecules/reagents able to discriminate between target molecules. Reagents with such features can be used in diverse settings like biological chemistry, diagnostics and in therapeutic applications. LÄS MER

7. 10. Engineering tissue – Bacterial cellulose as a potential biomaterial

   Författare :Maricris Esguerra; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bacterial cellulose Biocompatibility Blood vessels Non-neuronal cholinergic system Smooth muscle cells Tissue engineering;

   Sammanfattning : Cardiovascular diseases are still the predominant cause of death among the adult population in the Western world. Diseases such as myocardial infarction and stroke are caused by occluded blood vessels, as a result of atherosclerosis. LÄS MER