Sökning: "Bacterial Display"

Visar resultat 21 - 25 av 69 avhandlingar innehållade orden Bacterial Display.

  1. 21. Affibody ligands in immunotechnology applications

    Författare :Jenny Rönnmark; KTH; []
    Nyckelord :phage display; combinatorial; affinity; IgA ligand; immunohistochemistry; affibody-fusions;

    Sammanfattning : This thesis describes the development and use ofnon-immunoglobulin affinity proteins denoted affibodies asalternatives to antibodies in different immunotechnologyapplications. A 58 aa IgG Fc binding three-helix bundle domainZ, derived from staphylococcal protein A has been used asframework for library constructions, in which the face of themolecule involved in the native binding activity has beenengineered by combinatorial protein engineering. LÄS MER

  3. 22. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

    Författare :Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

    Sammanfattning : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. LÄS MER

  4. 23. Bifunctional ADAPTs: Opportunity for serological Half-life extension and Targeted therapy

    Författare :Andreas Wisniewski; Sophia Hober; Masood Kamali-Moghaddam; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ABD; ADAPT; Autoimmune diseases; Cancer; Half-life extension; HRP; Phage Display; Protein Engineering; Targeted therapy; Biotechnology; Bioteknologi;

    Sammanfattning : Small engineered scaffold proteins (ESPs) gain more and more popularity as biological drugs, due to their specificity and applicability in diagnostics and therapy. Thanks to their high stability, low immunogenicity and low production cost, they present themselves as a promising alternative to the market-leading antibodies. LÄS MER

  5. 24. In vivo and in vitro evolution of molecular mechanisms - Importance in B cell development and phage display

    Författare :Nina Nilsson; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; B cell development and phage display; transplantation; serology; Immunology; Immunologi; serologi; Biology; Biologi;

    Sammanfattning : Bone marrow resident hematopoietic stem cells differentiate into all different types of lymphoid populations, there among mature naive B cells expressing IgM and IgD. During the maturation, the B cells are subjected to several steps of selection to ensure proper development of the antigen receptor. LÄS MER

  7. 25. Coiled coil cytoskeleton in cell architecture and osmotic stress response in Streptomyces

    Författare :Katsuya Fuchino; Molekylär cellbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Coiled coil protein; Streptomyces coelicolor; polar growth; osmotic-stress response; turgor;

    Sammanfattning : Coiled coil proteins emerge as important determinants of bacterial cellular organization. Several such proteins display properties characteristic to metazoan intermediate filament (IF) proteins, and are therefore designated bacterial IF-like proteins. LÄS MER