Sökning: "Bacterial Display"

Visar resultat 16 - 20 av 69 avhandlingar innehållade orden Bacterial Display.

1. 16. Development of molecular recognition by rational and combinatorial engineering

   Författare :Andreas Jonsson; Stefan Ståhl; Mats Persson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Affibody molecule; albumin binding domain; protein engineering; phage display; amyloid beta peptide; TNF; HSA; Molecular biology; Molekylärbiologi;

   Sammanfattning : Combinatorial protein engineering, taking advantage of large libraries of protein variants and powerful selection technology, is a useful strategy for developing affinity proteins for applications in biotechnology and medicine. In this thesis, two small affinity proteins have been subjected to combinatorial protein engineering to improve or redirect the binding. LÄS MER

3. 17. Recombinant Antibodies – Tools for building proteomic platforms

   Författare :Anna Säll; Institutionen för immunteknologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody development; recombinant scFv antibodies; phage display; affinity proteomics; mass spectrometry;

   Sammanfattning : This thesis, which is based on four original papers, covers different aspects of the evolvement and development of proteomic platforms and the construction of human synthetic antibody libraries for the isolation of antibody reagents has been very central. There is an accelerating need for technologies capable of providing proteomic analysis of high sensitivity with good accuracy and an overall broad coverage as well as for biomarker validation. LÄS MER

4. 18. Cleavage Specificity of Mast Cell Chymases

   Författare :Mattias K. Andersson; Lars Hellman; Gunnar Pejler; Per Venge; Uppsala universitet; []
   Nyckelord :Biology; Immunology; Mast cell; Serine protease; Chymase; Cleavage specificity; Phage display; Biologi;

   Sammanfattning : Mast cells (MC) are potent inflammatory cells that are known primarily for their prominent role in IgE mediated allergies. However, they also provide beneficial functions to the host, e.g. in bacterial and parasitic defence. LÄS MER

5. 19. Perilipin expression in the islets of Langerhans and Surface expression in the baculovirus/insect cell system

   Författare :Jörgen Borg; Molekylär endokrinologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biology; Biologi; lipotoxicity; beta-cell; perilipin; amino-terminal anchor; Baculovirus; surface display;

   Sammanfattning : Surface display in insect cells: Systems available for surface display that utilize bacterial phages are insufficient for displaying a number of eukaryotic proteins. By instead presenting proteins on the surface of eukaryotic cells or virus, some of these problems might be overcome. LÄS MER

7. 20. Multifunctional Nanocellulose Composite Materials

   Författare :Olof Eskilson; Daniel Aili; Robert Selegård; Erik Martinsson; Aji Mathew; Linköpings universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; nanomaterials; nanocomposites; bacterial cellulose; wound care; plasmonics; catalysis;

   Sammanfattning : Nanoparticles (NPs) are particles with more than one dimension between 1 and 100 nm. Because of their small size, they typically display different physical and chemical properties than the corresponding bulk materials. NPs have been used in many different applications, such as in electronics, optics, catalysis, and in biomedicine. LÄS MER