Sökning: "single cell resolution"

Visar resultat 1 - 5 av 192 avhandlingar innehållade orden single cell resolution.

  1. 1. Genetic mechanisms regulating proliferation and cell specification in the Drosophila embryonic CNS

    Författare :Shahrzad Bahrampour; Stefan Thor; Jan-Ingvar Jönsson; Simon Sprecher; Linköpings universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

    Sammanfattning : The central nervous system (CNS) consists of an enormous number of cells, and large cellular variance, integrated into an elaborate network. The CNS is the most complex animal organ, and therefore its establishment must be controlled by many different genetic programs. LÄS MER

  3. 2. Miniaturised Microwell-based Cell Assays

    Författare :Emilie Weibull; Helene Andersson Svahn; Albert van den Berg; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; microwell; miniaturisation; high-resolution imaging; high-throughput; cell culture; single-cell; clone; heterogeneity; antibiotic susceptibility testing; concentration gradient; interfacing; microchannels; cell retrieval;

    Sammanfattning : Cell heterogeneity in genetically identical cell populations is becoming a well-known and important phenomenon in cell biology. Current methods commonly utilise population-based analysis founded on averaged result. Hence there is a need for high-throughput cell assays on the single-cell level. LÄS MER

  4. 3. Live Single Cell Imaging and Analysis Using Microfluidic Devices

    Författare :Mohammad Ali Khorshidi; Helene Andersson-Svahn; Håkan Jönsson; Carolina Wählby; Johan Elf; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Single cell analysis; time-lapse fluorescence imaging; automated image analysis; microwell; droplet microfluidics; NK cells; single cell tracking; migration behavior analysis; cell-cell interaction; optical microscopy; image analysis; image processing; microfluidics; immune cells; tracking; counting; morphology analysis;

    Sammanfattning : Today many cell biological techniques study large cell populations where an average estimate of individual cells’ behavior is observed. On the other hand, single cell analysis is required for studying functional heterogeneities between cells within populations. LÄS MER

  5. 4. Application of Proximity Ligation Assay for Multidirectional Studies on Transforming Growth Factor-β Pathway

    Författare :Agata Zieba; Ola Söderberg; Kathleen C. Flanders; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation; TGF-β signaling; Smad; single cell analysis; cell cycle; context-dependent signaling; CRC; Molekylär cellbiologi; Molecular Cellbiology; Medicinal Chemistry; Läkemedelskemi; Molecular Biology; Molekylärbiologi;

    Sammanfattning : A comprehensive understanding of how the body and all its components function is essential when this knowledge is exploited for medical purposes. The achievements in biological and medical research during last decades has provided us with the complete human genome and identified signaling pathways that governs the cellular processes that facilitates the development and maintenance of higher order organisms. LÄS MER

  7. 5. Spatially resolved and single cell transcriptomics

    Författare :Fredrik Salmén; Joakim Lundeberg; Patrik Ståhl; Elaine R. Mardis; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; spatial; transcriptomics; single cell; 3D; RNA-sequencing; Bioteknologi; Biotechnology;

    Sammanfattning : In recent years, massive parallel sequencing has revolutionized the field of biology and has provided us with a vast number of new discoveries in fields such as neurology, developmental biology and cancer research. A significant area is deciphering gene expression patterns, as well as other aspects of transcriptome information, such as the impact of splice variants and mutations on biological functions and disease development. LÄS MER