Sökning: "in vitro screening assay"

Visar resultat 1 - 5 av 60 avhandlingar innehållade orden in vitro screening assay.

1. 1. Creating cell microenvironments in vitro

   Författare :Patric Wallin; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cell migration; lipid bilayers; cell differentiation; liquid handling; surface functionalization; Microfluidics; chemotractant gradients; mammalian cell culture; cell microenvironments; in vitro screening assay;

   Sammanfattning : Stem cells have a great potential to bring about advancements in fields like developmental biology, drug discovery, cancer biology and tissue engineering. In order to be able to use stem cells to their full potential, it is important to have control over their behavior. LÄS MER

3. 2. Towards High-Throughput Phenotypic and Systemic Profiling of in vitro Growing Cell Populations using Label-Free Microscopy and Spectroscopy : Applications in Cancer Pharmacology

   Författare :Obaid Aftab; Mats Gustafsson; Neil Carragher; Uppsala universitet; []
   Nyckelord :label free vesicle detector; high-throughput; phase contrast microscopy; Library of Pharmacologically Active Compounds; High Content Screening; fluorometric microculture cytotoxicity assay; nuclear magnetic resonance; mass spectrometry;

   Sammanfattning : Modern techniques like automated microscopy and spectroscopy now make it possible to study quantitatively, across multiple phenotypic and molecular parameters, how cell populations are affected by different treatments and/or environmental disturbances. As the technology development at the instrument level often is ahead of the data analytical tools and the scientific questions, there is a large and growing need for computational algorithms enabling desired data analysis. LÄS MER

4. 3. In vitro and in vivo approaches in the characterization of XTH gene products

   Författare :Nomchit Kaewthai; Harry Brumer; Jocelyn Rose; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Arabiodopsis thaliana; Hordeum vulgare; plant cell wall; xyloglucan; glycoside hydrolase family 16; xyloglucan endo-transglycosylase hydrolase gene family; xyloglucan endo-hydrolase; heterologous protein expression; Pichia pastoris; T-DNA insertion; in situ XET XEH assay; high-througput screening; Plant physiology; Växtfysiologi; Biochemistry; Biokemi; Functional genomics; Funktionsgenomik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : ABSTRACT The xyloglucan endo-transglycosylase/hydrolase (XTH) genes are found in all vascular and some nonvascular plants. The XTH genes encode proteins which comprise a subfamily of glycoside hydrolase (GH) family 16 in the Carbohydrate-Active enZYmes (CAZY) classification. LÄS MER

5. 4. Advanced molecular tools for diagnostic analyses of RNA and antibodies in situ and in solution

   Författare :Mengqi Wang; Ulf Landegren; Anders Ståhlberg; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Homogenous serological assay; PCR-based antibody detection; Proximity extension assay; SARS-CoV-2 antibody; Extracellular vesicles; Proximity barcoding assay; in situ superRCA; mutant transcript;

   Sammanfattning : Advanced molecular diagnostics uses in vitro biological assays to detect nucleic acids or proteins even in low concentrations across samples, allowing for the identification of biomarkers, monitoring the course of the disease over time, and selection of appropriate therapy. In this thesis, I focus on development and early applications of several molecular tools of expected value in research, and eventually also clinically. LÄS MER

7. 5. Chemically Induced DNA Damage in Extended-term Cultures of Human Lymphocytes

   Författare :Maria Andersson; Björn Hellman; Leif Busk; Uppsala universitet; []
   Nyckelord :Toxicology; Comet assay; DNA-damaging agents; DNA repair; Extended-term Cultures of Human Lymphocytes; Genotoxicity; Induced DNA damage; In vitro; Mouse Lymphoma Cells; Primary Cell Cultures; Reactive Oxygen Species; Toxikologi;

   Sammanfattning : Generation of DNA damage is regarded to be an important initial event in the development cancer. Consequently, a battery of tests have been developed to detect different types of genotoxic effects in order to be able to predict the potential genotoxicity and mutagenicity of chemicals, including both pharmaceutical drugs and various types of environmental and occupational agents, as well as dietary factors. LÄS MER