Sökning: "Pichia pastoris"

Visar resultat 1 - 5 av 53 avhandlingar innehållade orden Pichia pastoris.

  1. 1. Heterologous expression and characterization of xyloglucan endotransglycosylases from Populus tremula x tremuloides

    Författare :Åsa Kallas; KTH; []
    Nyckelord :xyloglucan endotransglycosylase; transglycosylation; hybrid aspen; cell expansion; heterologous expression; Pichia pastoris; N-glycosylation; X-ray crystallography;

    Sammanfattning : Xyloglucan endotransglycosylases (XETs) are enzymes involvedin the reorganization of the primary cell wall. This thesisdescribes several aspects of XET16A from hybrid aspen (Populustremula x tremuloides). The gene was isolated from the hybridaspen cDNA library and the protein was expressed heterologouslyin the methylotrophic yeast Pichia pastoris. LÄS MER

  3. 2. Expression and Mutagenesis studies of Candida antactica lipase B

    Författare :Johanna C. Rotticci-Mulder; KTH; []
    Nyckelord :Protein expression; Candida antarctica lipase B; Pichia pastoris; site-directed mutagenesis; fermentation; selection;

    Sammanfattning : Recombinant Candida antarctica lipase B was successfullyproduced in the methylotropic yeast Pichia pastoris. Thespecific activities of Candida antarctica lipase B produced inPichia pastoris and commercial Candida antarctica lipase B fromNovozymes were the same. In shake-flask cultivations theexpression levels were about 25 mg L-1. LÄS MER

  4. 3. Exploring Unorthodox Aquaporins : Characterization of NbXIPs & AtNIPs

    Författare :Henry Ampah-Korsah; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Aquaporins; XIPs; NIPs; Phosphorylation; Pichia pastoris; Overexpression; boric acid; membrane proteins;

    Sammanfattning : AbstractAquaporins are membrane integral proteins that facilitate the transport of waterand/or other small neutral molecules across biological membranes in cells in allforms of life. Among the subfamilies of aquaporins in higher plants, the X IntrinsicProteins (XIPs) are the most recently discovered subfamily and the leastcharacterized. LÄS MER

  5. 4. Recombinant Enzymes in Pyrosequencing Technology

    Författare :Nader Nourizad; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

    Sammanfattning : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. LÄS MER

  7. 5. Expression and Function of Human and Plant Aquaporins

    Författare :Maria Agemark; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aquaporin; water channel; overexpression; Pichia pastoris; AQP8; AQP5; SoPIP2; 1;

    Sammanfattning : The aquaporins (AQPs) belong to a family of water permeable membrane channels found in virtually all living organisms. Thirteen isoforms of mammalian AQPs are found, whereas in Arabidopsis thaliana 35 genes encoding AQPs are found. The AQPs are distributed in different organs, cell types and in different subcellular membranes. LÄS MER