Sökning: "Reverse transcription"

Visar resultat 21 - 25 av 95 avhandlingar innehållade orden Reverse transcription.

  1. 21. Development of adenoviral vectors for monitoring telomerase activity in living cells

    Författare :Anna Edqvist; Neurokirurgi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cytogenetics; Genetics; cancer; onkologi; Cytologi; cancerology; oncology; Cytology; GBM; HSC; adenovirus; hTERT; Genetik; cytogenetik;

    Sammanfattning : ABSTRACT Telomerase activity is a potential molecular marker for cancer and primitive cells in regenerative tissues. The most commonly used method to measure telomerase activity in cells is the telomerase reverse amplification protocol (TRAP) assay, where the protein extracts derived from homogenized cells are analysed. LÄS MER

  3. 22. G protein-coupled estrogen receptor 1 (GPER) in the female reproductive tract: from physiology to cancer

    Författare :Zuzana Kolkova; Lund Obstetrik och gynekologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

    Sammanfattning : Estrogen effects are mediated either through genomic action involving the classical estrogen receptors, estrogen receptor alpha (ERα) and beta (ERß), which function as transcription factors in the nucleus, or through rapid non-genomic action via receptors associated with plasma membrane. Recently, a member of the G protein-coupled receptor family was ascribed estrogen receptor properties and proposed as a candidate for mediating non-genomic estrogen signaling. LÄS MER

  4. 23. Structure and Dynamics of the Hepatitis B Virus Encapsidation Signal Revealed by NMR Spectroscopy

    Författare :Sara Flodell; Stephan Grzesiek; Umeå universitet; []
    Nyckelord :HBV; RNA; isotope labelling; NMR; structure determination; medicinsk biokemi; Medical Biochemistry;

    Sammanfattning : This thesis describes the study of the three-dimensional structure and dynamics of the hepatitis B virus (HBV) encapsidation signal, epsilon, by means of nuclear magnetic resonance (NMR) and mutational data. HBV replicates by reverse transcription of an RNA pregenome into the viral DNA genome, which becomes enclosed in viral particles (encapsidation). LÄS MER

  5. 24. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies

    Författare :Amal Elfaitouri; Jonas Blomberg; Helene Norder; Uppsala universitet; []
    Nyckelord :Microbiology; Real-time PCR; broadly targeted PCR; degenerated PCR; rTth DNA polymerase; thermolabile UNG; QPIA; IgM; Mikrobiologi;

    Sammanfattning : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. LÄS MER

  7. 25. Studies on the mechanisms of carcinogenesis and cancer cell death

    Författare :Qiao Li; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Gastric cancer is the second most common cause of cancer-related mortality in the world. The mechanisms of gastric carcinogenesis are not fully elucidated. LÄS MER