Sökning: "Reverse transcription"

Visar resultat 11 - 15 av 95 avhandlingar innehållade orden Reverse transcription.

1. 11. Engineering cytosolic acetyl-CoA metabolism in Saccharomyces cerevisiae

   Författare :Yiming Zhang; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; reverse engineering; flavodoxin; genomic DNA sequencing; ferredoxin; histone deacetylase; adaptive evolution; ferredoxin flavodoxin NADP reductase; pyruvate decarboxylase; citrate synthase; hexose transporter; yeast; mitochondria; aerobic growth; metabolic engineering.; acetyl-CoA; central carbon metabolism;

   Sammanfattning : A Saccharomyces cerevisiae strain carrying deletions in all three pyruvate decarboxylase genes (also called Pdc negative yeast) represents a non-ethanol producing platform strain for biochemical production. However, it cannot grow on glucose as the sole carbon source due to the lack of cytosolic acetyl-CoA for lipid biosynthesis. LÄS MER

3. 12. Telomerase reverse transcriptase (TERT) : promoter mutation and novel function in human cancer

   Författare :Tiantian Liu; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Telomerase reverse transcriptase (TERT) is the catalytic component of telomerase, an RNAdependent DNA polymerase responsible for telomere elongation. TERT is transcriptionally repressed in most normal human somatic cells with limited life-span, which contributes to telomerase silence. LÄS MER

4. 13. HIV-1 reverse transcriptase as a target in the development of specific enzyme inhibitors

   Författare :Hong Zhang; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Human immunodeficiency virus type I (HIV-I), which causes the acquired immunodeficiency syndrome (AIDS), begins its intracellular infection life cycle with reverse transcription of its plus-strand RNA genome into a double-stranded proviral DNA intermediate which is integrated into the host chromosome inducing a persistent infection. A virally encoded enzyme, reverse transcriptase (RT), carries out the reverse transcription process by performing all three enzymatic activities, i. LÄS MER

5. 14. The role of telomerase reverse transcriptase in human malignancies : genetic polymorphisms and promoter mutations

   Författare :Xiaotian Yuan; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Telomerase is a ribonucleoprotein with its catalytic subunit telomerase reverse transcriptase (TERT) as a key component, lengthening telomeres. In differentiated human cells, telomerase is silent due to the transcriptional repression of the TERT gene, but activated in oncogenesis. LÄS MER

7. 15. Reverse transcriptase assay for analysis of resistance to anti-HIV drugs and their mechanism of action

   Författare :Xingwu Shao; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :HIV-1; RT; antiviral drugs; NRTls; NNRTls; drug resistance; viral load;

   Sammanfattning : Reverse transcriptase (RT) is a viral enzyme and one of the main targets for drugs against human immunodeficiency virus (HIV). This work presents a set of sensitive non-radioactive RT assays were developed: standard RT inhibition assay (IC assay); RT binding inhibition assay; RTprotein detection assay and template-primer destruction assay or chain termination assay (CT assay). LÄS MER