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Visar resultat 1 - 5 av 35 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Improved Techniques for Protein Analysis Focusing on Membrane Proteins and Hydrophobic Peptides

   Författare :Theres Redeby; Åsa Emmer; Jonas ergquist; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Integral membrane proteins; Hydrophobic peptides; Sample handling; MALDI-TOF-MS; Structured sample support; CE; fraction collection; microchannel; Analytical chemistry; Analytisk kemi;

   Sammanfattning : In this thesis, the vital cell functions performed by integral membrane proteins (IMPs) are briefly discussed. Such proteins are under-represented in most protein studies due to the hydrophobic nature of IMPs, which seriously complicate their solubilization, sample handling, preparation, separation and analysis. LÄS MER

3. 2. Improved techniques for CE-MALDI-MS off-line coupling and MALDI-MS analysis of primarily hydrophobic proteins and peptides

   Författare :Johan Jacksén; Åsa Emmer; Anna Karin/Minna Borg Karlsson/Hakkarainen; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon microcanal; Matrix; Protein cleavage digestion.; Kapillärelektrofores; Hydrofoba peptider; Matris-assisterad laserdesorptions-joniserings-masspektrometri; Strukturbelagd provplatta; Off-line interface; Mikrokanal i kisel; Matris; Proteinklyvning -spjälkning.; Analytical chemistry; Analytisk kemi;

   Sammanfattning : Due to the hydrophobic nature of integral membrane proteins (IMP) they give rise to several difficulties concerning handling and analysis, which is not the case for the most water soluble proteins. New analysis methods are needed, where the insolubility problems of the hydrophobic proteins due to aggregation and adhesion are tackled. LÄS MER

4. 3. Integral membrane protein MALDI-MS analysis - Sample handling and fabrication of a structured target

   Författare :Theres Redeby; KTH; []
   Nyckelord :Integral membrane proteins; Hydrophobic peptides; Sample handling; MALDI-MS; Structured sample support;

   Sammanfattning : .... LÄS MER

5. 4. HYDROPHOBIC PEPTIDE TAGS - As tools in bioseparation and investigation of recombinant proteins

   Författare :Sara Fexby; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; E. coli; Biochemical technology; Biokemisk teknik; fusion protein; tyrosine; hydrophobicity; Hydrophobic interaction chromatography; aqueous two-phase system;

   Sammanfattning : This thesis describes the possibility of using hydrophobic peptide tags as tools for bioseparation and for the investigation of recombinant proteins. The hydrophobicity of a protein can easily be changed by minor modifications, such as fusions of tyrosine-containing peptides. LÄS MER

7. 5. On the adsorption behaviour of saliva and purified salivary proteins at solid/liquid interfaces

   Författare :Liselott Lindh; Malmö högskola; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; acidic proline-rich proteins; adsorption; BSM; diffusion; ellipsometry; interfaces; mass transport limitation; mucins; MUC5B; saliva; salivary proteins; statherin; surface properties;

   Sammanfattning : Den salivfilm som bildas genom selektiv proteinadsorption på ytor i munhålan har stor profylaktisk betydelse genom sin skyddande funktion och befrämjar därigenom oral hälsa. Några av de adsorberade komponenterna har smörjande funktion (t ex muciner, sura prolinrika proteiner (PRP) och statherin), som är viktig bl a för att förhindra slitage mellan tandytor vid tuggning och hos gnisslare. LÄS MER