Sökning: "single-cell sequencing"
Visar resultat 1 - 5 av 161 avhandlingar innehållade orden single-cell sequencing.
1. Technologies for Single Cell Genome Analysis
Sammanfattning : During the last decade high throughput DNA sequencing of single cells has evolved from an idea to one of the most high profile fields of research. Much of this development has been possible due to the dramatic reduction in costs for massively parallel sequencing. LÄS MER
2. Spatially resolved and single cell transcriptomics
Sammanfattning : In recent years, massive parallel sequencing has revolutionized the field of biology and has provided us with a vast number of new discoveries in fields such as neurology, developmental biology and cancer research. A significant area is deciphering gene expression patterns, as well as other aspects of transcriptome information, such as the impact of splice variants and mutations on biological functions and disease development. LÄS MER
3. Single-cell methodologies for ecological and metabolic mapping of mixotrophic microeukaryotes
Sammanfattning : Mixotrophy in aquatic protists is pivotal for our understanding of aquatic microbial food web dynamics. This thesis is centered around aquatic unicellular mixotrophs, and comprises three methodological approaches aimed to tackle mixotroph ecology at single-cell resolution: the identification of actively feeding mixotrophs in natural samples, the determination of specific interactions among mixotrophs and bacterial prey, and the profiling of two distinct mixotrophic populations based on the gene expression of their constitutive individuals. LÄS MER
4. Variational methods for phylogeny and single-cell genomics
Sammanfattning : The investigation of the evolutionary history of organisms, both at the cellular level and at the species level, is a relevant research topic in computational biology. These investigations lead to a deeper understanding of developmental history, cancer progression, the genetic similarity of species, and more. LÄS MER
5. Detection and Sequencing of Amplified Single Molecules
Sammanfattning : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). LÄS MER