Sökning: "membrane protein topology"

Visar resultat 1 - 5 av 58 avhandlingar innehållade orden membrane protein topology.

1. 1. The Ins and Outs of Membrane Proteins : Topology Studies of Bacterial Membrane Proteins

   Författare :Mikaela Rapp; Gunnar von Heijne; Christopher Tate; Stockholms universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; membrane protein; topology; dual topology; Biochemistry; Biokemi;

   Sammanfattning : α-helical membrane proteins comprise about a quarter of all proteins in a cell and carry out a wide variety of essential cellular functions. This thesis is focused on topology analyses of bacterial membrane proteins. The topology describes the two-dimensional structural arrangement of a protein relative to the membrane. LÄS MER

3. 2. Engineering membrane proteins for production and topology

   Författare :Stephen Toddo; Daniel Daley; Roslyn Bill; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Membrane protein; Over-expression; Protein production; Codon optimization; Escherichia coli; AraH; NarK; mRNA secondary structure; coding sequence; ribosome binding site; RBS; topology; split-GFP; Biochemistry; biokemi;

   Sammanfattning : The genomes of diverse organisms are predicted to contain 20 – 30% membrane protein encoding genes and more than half of all therapeutics target membrane proteins. However, only 2% of crystal structures deposited in the protein data bank represent integral membrane proteins. LÄS MER

4. 3. GFP as a tool to monitor membrane protein topology and overexpression in Escherichia coli

   Författare :David Drew; Jan-Willem de Gier; Gunnar von Heijne; Bert Poolman; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; GFP; membrane protein; topology; overexpression; high-throughput; Biochemistry; Biokemi;

   Sammanfattning : Membrane proteins are essential for life, and roughly one-quarter of all open reading frames in sequenced genomes code for membrane proteins. Unfortunately, our understanding of membrane proteins lags behind that of soluble proteins, and is best reflected by the fact that only 0. LÄS MER

5. 4. Determinants of membrane protein topology

   Författare :Marie Johansson; Collin Robinson; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; biokemi; Biochemistry;

   Sammanfattning : The living cell, no matter if it is a simple prokaryotic bacterial cell or a highly specialised eukaryotic cell, synthesises a vast number of different proteins. In most organisms, 20-30 % of all proteins are thought to be transmembrane integral membrane proteins. LÄS MER

7. 5. Sequence-based predictions of membrane-protein topology, homology and insertion

   Författare :Andreas Bernsel; Gunnar von Heijne; Volkhard Helms; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; membrane protein; topology prediction; hidden markov model; homology detection; Sec translocon; Bioinformatics; Bioinformatik; Biochemistry; biokemi;

   Sammanfattning : Membrane proteins comprise around 20-30% of a typical proteome and play crucial roles in a wide variety of biochemical pathways. Apart from their general biological significance, membrane proteins are of particular interest to the pharmaceutical industry, being targets for more than half of all available drugs. LÄS MER