Sökning: "Codon optimization"

Visar resultat 1 - 5 av 10 avhandlingar innehållade orden Codon optimization.

  1. 1. Engineering membrane proteins for production and topology

    Författare :Stephen Toddo; Daniel Daley; Roslyn Bill; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Membrane protein; Over-expression; Protein production; Codon optimization; Escherichia coli; AraH; NarK; mRNA secondary structure; coding sequence; ribosome binding site; RBS; topology; split-GFP; Biochemistry; biokemi;

    Sammanfattning : The genomes of diverse organisms are predicted to contain 20 – 30% membrane protein encoding genes and more than half of all therapeutics target membrane proteins. However, only 2% of crystal structures deposited in the protein data bank represent integral membrane proteins. LÄS MER

  3. 2. Optimization of suicide gene therapy in stem cell transplantation with special reference to the selection marker OuaSelect

    Författare :Alexandra Treschow; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Gene therapy; cell selection; suicide gene; cancer; immunotherapy; viral vectors; thymidine kinase; Na ; K -ATPase;

    Sammanfattning : For many patients suffering from leukemia and other hematological malignancies, allogeneic hematopoietic stem cell transplantation (HSCT) represents the only curative approach. However, an optimal HSCT procedure is yet to be developed. Transplant-related morbidity and mortality are severe complications arising from this treatment modality. LÄS MER

  4. 3. Development of vaccines and experimental models for chronic infections caused by the hepatitis C virus

    Författare :Lars Frelin; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :HCV; chronic infection; DNA vaccine; NS3; viral vectors; transgenic mice;

    Sammanfattning : The hepatitis C virus (HCV) is a major cause of chronic liver disease worldwide. It is estimated that HCV affects approximately 170 million people around the world. One feature of HCV infection is the high rate of viral persistence. LÄS MER

  5. 4. Human Aquaporins: Production, Characterization and Interactions

    Författare :Jennie Sjöhamn; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aquaporins; membrane protein; protein production; protein characterization; protein:protein interactions; structural biochemistry;

    Sammanfattning : Membrane proteins are essential components of the cell and responsible for the communication with the outside environment and transport of molecules across the membrane. Water transport is facilitated by aquaporins, which are water selective transmembrane pores that serve to maintain cell homeostasis. LÄS MER

  7. 5. Improving immunogenicity of hepatitis B and C viral proteins for vaccine design

    Författare :Antony Chen; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The hepatitis B and C virus (HBV, HCV) both presents a global health challenge, with an estimated 350 million individuals chronically infected with HBV and over 200 million infected with HCV. Reported cases of HBV and HCV have declined significantly due to screening of blood products and the availability of HBV vaccination. LÄS MER