Sökning: "electron cryo-microscopy"

Hittade 4 avhandlingar innehållade orden electron cryo-microscopy.

  1. 1. Structural investigation of human mitochondrial translation and off-target antibiotic binding

    Författare :Vivek Singh; Alexey Amunts; Yaser Hashem; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Mitochondrial translation; mitoribosome; electron cryo-microscopy; LRPPRC-SLIRP; antibiotics; streptomycin; Biophysics; biofysik;

    Sammanfattning : Human mitochondrial translation machinery has evolved to translate 13 mitochondrial mRNAs encoding components of the oxidative phosphorylation pathway responsible for ATP production. The structural basis of human mitochondrial translation is distinct from the canonical bacterial and cytosolic translation systems. LÄS MER

  3. 2. Structural characterisation of mitochondrial macromolecular complexes using cryo-EM : Mitoribosome biogenesis and respiratory chain supercomplex

    Författare :Sorbhi Rathore; Martin Ott; Daniel Wilson; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Mitochondria; mitoribosome biogenesis; mitoribosome assembly factors; yeast respiratory supercomplexes; single particle electron cryo-microscopy; cryo-EM sample preparation; Biochemistry; biokemi;

    Sammanfattning : Mitochondria, popularly known as powerhouse of the cell, contain specialised mitoribosomes that synthesise essential membrane proteins. These essential proteins are required to form enzyme complexes, which carry out the process of oxidative phosphorylation (OXPHOS). LÄS MER

  4. 3. Cryo-EM Studies of Macromolecular Complexes from Photosynthetic Organisms

    Författare :Annemarie Perez Boerema; Alexey Amunts; Mei Li; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cryo-EM; membrane proteins; photosynthesis; translation; chloroplast; ribosome; photosystem I; protein structure; Biochemistry; biokemi;

    Sammanfattning : Plants, algae, and cyanobacteria convert light energy into chemical energy through the process of photosynthesis, fueling the planet and making life as we know it possible. Photosystem I (PSI) is one of the main photosynthetic complexes, responsible for this process. LÄS MER

  5. 4. The dynamic envelope of a fusion class II virus : molecular reorganizations during prefusion stages of Semliki forest virus

    Författare :Lars Haag; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Alphavirus; SFV; fusion; cryo-EM; formaldehyde; reorganization; cross-link; 3D reconstruction; acid pH; Pepscan; MAb; inactivation; dissociation; monoclonal antibody;

    Sammanfattning : The aim of this study was to explore a membrane fusion mechanism, prevailing in alphaviruses and known as virus class 11 fusion. The model virus for this mechanism is Semliki Forest virus (SFV) of the alphavirus family. LÄS MER