Sökning: "aqueous two phase partitioning"

Visar resultat 1 - 5 av 26 avhandlingar innehållade orden aqueous two phase partitioning.

1. 1. Protein Surfaces Probed by Aqueous Two-phase Partitioning

   Författare :Kristina Berggren; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; partitioning; Aqueous two-phase systems; protein surface; peptide; Biochemistry; amino acid residue; Biokemi;

   Sammanfattning : This thesis is concerned primarily with peptide and protein partitioning in aqueous two-phase systems and the connection between partitioning behavior and protein structure. The surface properties of a protein is often crucial for recognition and interaction with other molecules. LÄS MER

3. 2. Partitioning of biomolecules in thermoseparating polymer-water two-phase systems

   Författare :Hans-Olof Johansson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aqueous two-phase system; temperature-induced phase separation; amino acid and peptide separation; polymer; partitioning; Biology; Biologi;

   Sammanfattning : The partitioning behaviour of different types of molecules in thermoseparating two-phase systems has been studied. A thermoseparating two phase system was formed by heating a water solution, which contained a thermoseparating polymer, above the cloud point temperature. LÄS MER

4. 3. Peptide-tagged proteins in aqueous two-phase systems

   Författare :Anna Nilsson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; Biokemi; metabolism; Biochemistry; green fluorescent protein; cutinase; tryptophan; Aqueous two-phase system; peptide-tagged protein;

   Sammanfattning : This thesis deals with proteins containing peptide tags for improved partitioning in aqueous two-phase systems. Qualitatively the peptide-tagged protein partitioning could be predicted from peptide data, i.e. partitioning trends found for peptides were also found for the peptide-tagged proteins. LÄS MER

5. 4. Lactic acid production: extractive fermentation in aqueous two-phase systems

   Författare :Jordi Planes; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; gel excludion chromatography; polyethylenimine; ethylene oxide propylene oxide; phase forming polymers; aqueous two-phase systems; extractive fermentation; Lactococcus; Lactobacillus; Mikrobiologi; bakteriologi; virologi; mykologi; bacteriology; virology; mycology; Microbiology;

   Sammanfattning : There is an increased interest in the microbial production of lactic acid as a raw material for the synthesis of lactic acid polymers as biodegradable plastics. However, two factors limit the economical competitiveness of lactic acid production by microorganisms: lactic acid inhibition, which decreases both fermentation rate and the maximum lactic acid concentration, and the efficiency in the recovery of lactic acid. LÄS MER

7. 5. Separation Methods in Membrane Proteomics -Integration of Aqueous Two-Phase Partitioning for Enrichment and Pre-Fractionation

   Författare :Henrik Everberg; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Membrane proteins; Proteins; metabolism; Biokemi; Metabolism; Biochemistry; Aqueous two-phase partitioning; enzymology; Proteiner; enzymologi; Proteomics;

   Sammanfattning : The study of the protein complement of a genome is called proteomics. Proteomics can be defined as identification, quantification and characterization of a total set of proteins expressed at different time-points or under different cellular states to provide global information about biological processes. LÄS MER