Sökning: "affinity proteins"
Visar resultat 1 - 5 av 570 avhandlingar innehållade orden affinity proteins.
1. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins
Sammanfattning : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). LÄS MER
2. Selective extraction systems for detection and purification of membrane proteins
Sammanfattning : Increased understanding of membrane proteins is important for characterization of biochemical processes. Improved membrane protein isolation methods is a key issue for effective functional and structural determination of the large amount of unknown membrane proteins. LÄS MER
3. Generation and engineering of ABD-derived affinity proteins for clinical applications
Sammanfattning : Proteins that specifically recognize and bind to other molecules or structures are important tools in industrial and medical applications. Binding proteins engineered from small stable scaffold proteins have been utilized for several purposes due to their favorable biophysical properties, tolerance to mutagenesis, efficient tissue penetration and ease of production. LÄS MER
4. Expression and purification of membrane proteins: Focus on the G-protein coupled receptor MC4r
Sammanfattning : Membrane proteins are crucial components of the cell and are involved in many biological processes. Recombinant overexpression systems together with different purification methods are necessary to obtain large amounts of purified receptor for biophysical and functional studies. LÄS MER
5. Mass Spectrometry and Affinity Based Methods for Analysis of Proteins and Proteomes
Sammanfattning : Proteomics is a fast growing field and there has been a tremendous increase of knowledge the last two decades. Mass spectrometry is the most used method for analysis of complex protein samples. It can be used both in large scale discovery studies as well as in targeted quantitative studies. LÄS MER