Sökning: "Phage display technology"

Visar resultat 31 - 35 av 48 avhandlingar innehållade orden Phage display technology.

  1. 31. Generation and engineering of ABD-derived affinity proteins for clinical applications

    Författare :Sarah Lindbo; Sophia Hober; Christoph Rader; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ABD; ADAPT; affinity proteins; protein engineering; radionuclide molecular imaging; HER2; Bioteknologi; Biotechnology;

    Sammanfattning : Proteins that specifically recognize and bind to other molecules or structures are important tools in industrial and medical applications. Binding proteins engineered from small stable scaffold proteins have been utilized for several purposes due to their favorable biophysical properties, tolerance to mutagenesis, efficient tissue penetration and ease of production. LÄS MER

  3. 32. Characterization of Antigenic Properties and High Throughput Protein Purification

    Författare :Johanna Steen; Sophia Hober; Mats Ohlin; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; high throughput; protein purification; sample preparation; antigen solubility; immunogenicity; epitope mapping; antigen design; Bioengineering; Bioteknik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : To understand the cellular processes, knowledge of the localization and function of proteins are essential. There are several high throughput ventures examining the human proteome. However, there are some bottlenecks in these ventures. For example the production and expression of soluble proteins for analysis. LÄS MER

  4. 33. Staphylococcal surface display and its applications in bioscience

    Författare :Patrik Samuelson; KTH; []
    Nyckelord :;

    Sammanfattning : Display of heterologous proteins on the surface ofmicroorganisms, enabled by means of recombinant DNA technology,has become an increasingly used strategy in variousapplications in microbiology, biotechnology and vaccinology.This thesis presents two bacterial systems, designed forsurface display of chimeric proteins on the food-gradestaphylococcal speciesStaphylococcus xylosusandStaphylococcus carnosus, respectively. LÄS MER

  5. 34. Generation and evolution of human antibody fragments - The CDR shuffling approach

    Författare :Pernilla Jirholt; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; transplantation; serology; Immunology; Biokemisk teknik; Biokemi; Biochemistry; Biochemical technology; Metabolism; Immunologi; serologi;

    Sammanfattning : Antibodies are important proteins participating in a number of reactions to protect us from harmful diseases. Apart from this important function, they are also useful in different technical and medical applications. This, however, relies on our possibility to produce antibodies in vitro. LÄS MER

  7. 35. Engineering Antibody Specificity Using Combinatorial Antibody Libraries

    Författare :Jenny Boström; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; antibody Antigen Interactions; Dual Specific Antibodies; Herceptin; Human Epidermal Growth Factor Receptor 2; Thermodynamic Analysis; therapeutic Antibody Engineering; Synthetic Phagedisplayed Antibody Libraries; Vascular Endothelial Growth Factor; Bacterial Display; trastuzumab;

    Sammanfattning : The research presented in this thesis investigates antibody specificity, antibody-antigen and protein-protein interactions using combinatorial antibody libraries, phage-display and bacterial display technology. One goal was to develop a method to generate antibodies that bind two protein antigens with high affinity using a single-antigen binding site, and to investigate the molecular and thermodynamic requirements for antibody multispecificity. LÄS MER