Sökning: "Phage display technology"

Visar resultat 26 - 30 av 48 avhandlingar innehållade orden Phage display technology.

1. 26. Development of molecular recognition by rational and combinatorial engineering

   Författare :Andreas Jonsson; Stefan Ståhl; Mats Persson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Affibody molecule; albumin binding domain; protein engineering; phage display; amyloid beta peptide; TNF; HSA; Molecular biology; Molekylärbiologi;

   Sammanfattning : Combinatorial protein engineering, taking advantage of large libraries of protein variants and powerful selection technology, is a useful strategy for developing affinity proteins for applications in biotechnology and medicine. In this thesis, two small affinity proteins have been subjected to combinatorial protein engineering to improve or redirect the binding. LÄS MER

3. 27. Selection and use of affinity proteins developed by combinatorial engineering

   Författare :Kristofer Sandström; KTH; []
   Nyckelord :phage display; combinatorial; selection; cd28; cllulosome; cellulose;

   Sammanfattning : In affinity protein biotechnology the selective bindingbetween a chosen protein and an interacting biomolecule isutilized for a variety of applications including bioseparation,detection and therapy. Traditionally, affinity proteinsrecruited for such applications have been derived from naturalproteins or immunoglobulins generated via immunization routes. LÄS MER

4. 28. Functionally-associated target antigens in cancer. Defined by human antibodies and small interfering RNAs

   Författare :Johan Fransson; Institutionen för immunteknologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; phage display; Antibody; cancer; transplantation; Immunologi; serologi; serology; Immunology; onkologi; Cytologi; cancerology; oncology; Biochemistry; Metabolism; Biokemi; metabolism; Biotechnology; Bioteknik; Cytology;

   Sammanfattning : Cancer is the collective name for diseases that have one thing in common ? uncontrolled cell growth. The increase in cancer cell proliferation eventually results in tumor formation and, in the worst case, metastatic spread to other organs. Common treatment modalities of cancer include chemotherapy and radiation therapy. LÄS MER

5. 29. In vivo and in vitro evolution of molecular mechanisms - Importance in B cell development and phage display

   Författare :Nina Nilsson; Institutionen för immunteknologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; B cell development and phage display; transplantation; serology; Immunology; Immunologi; serologi; Biology; Biologi;

   Sammanfattning : Bone marrow resident hematopoietic stem cells differentiate into all different types of lymphoid populations, there among mature naive B cells expressing IgM and IgD. During the maturation, the B cells are subjected to several steps of selection to ensure proper development of the antigen receptor. LÄS MER

7. 30. Gliadin-Blocking Peptides In vitro assessment of their potential to alleviate celiac disease development

   Författare :Karolina Hoffmann; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; high-gliadin affinity blocking peptides; Caco-2 cells; in vitro digestion; tissue transglutaminase; celiac disease; gliadin; α-amylase trypsin inhibitors;

   Sammanfattning : This thesis work aimed to investigate the potential of synthetic peptides as agents to block wheat prolamins (gliadins) that trigger the development of symptoms in celiac disease. The first step of this investigation included selection and synthesis of peptides with high affinity to gliadins and assessment of the potential of these so-called blocking peptides to limit gliadin reactivity in vitro. LÄS MER