Sökning: "NATURAL SCIENCES Chemistry Biochemistry Structural biology"

Visar resultat 16 - 20 av 51 avhandlingar innehållade orden NATURAL SCIENCES Chemistry Biochemistry Structural biology.

  1. 16. In silico protein design for the enhancement of protein stability and function

    Författare :David A. Hueting; Per-Olof Syrén; Hjalmar Brismar; Zbynek Prokop; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Protein Engineering; Ancestral sequence reconstruction; terpene cyclases; SARS-CoV-2; Spike protein; PET; PETase; rational engineering; Proteinteknik; Förfäders sekvensrekonstruktion; Terpencyklas; SARS-CoV-2; Spike protein; PET; PETas; rationell proteinteknik; Kemi; Chemistry;

    Sammanfattning : Enzymes are natures catalysts that increase the rate of a chemical reaction. The increased rate of a reaction is required to be able to sustain life. Despite the huge impact of enzymes, they are not perfect catalysts. LÄS MER

  3. 17. Methods for elucidating membrane protein structure and function

    Författare :Carolina Trkulja; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; patch clamp; antibodies; mass spectrometry; ion channels; proteomics; fluorescence; TRPV1; cholesterol.; limited proteolysis; Membrane proteins;

    Sammanfattning : Elucidation of the structure-function relationship of membrane proteins increases our understanding of many biological processes, both physiological and pathological. However, membrane proteins have been notoriously difficult to study, having part of their structure embedded into the lipid cell membrane. LÄS MER

  4. 18. Calculations of Reaction Mechanisms and Entropic Effects in Enzyme Catalysis

    Författare :Masoud Kazemi; Johan Åqvist; Per E. M. Siegbahn; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Enzyme catalysis; Entropy; Cytidine deamination; Ribosome; Peptidyl-tRNA hydrolysis; Peptide bond formation; Empirical valence bond method; Density functional theory; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi; Biokemi; Biochemistry; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Sammanfattning : Ground state destabilization is a hypothesis to explain enzyme catalysis. The most popular interpretation of it is the entropic effect, which states that enzymes accelerate biochemical reactions by bringing the reactants to a favorable position and orientation and the entropy cost of this is compensated by enthalpy of binding. LÄS MER

  5. 19. Into the Membrane and Beyond : Improving Membrane Protein Over-Expression in Escherichia coli

    Författare :Mirjam Klepsch; Jan-Willem de Gier; Reinhard Grisshammer; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; membrane protein over-expression; proteomics; T7 RNA polymerase; peptide transport; strain engineering; Biochemistry; Biokemi; Biophysical chemistry; Biofysikalisk kemi; biokemi; Biochemistry;

    Sammanfattning : Membrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. LÄS MER

  7. 20. Computational Modeling of the Structure, Function and Dynamics of Biomolecular Systems

    Författare :Yashraj Kulkarni; Shina Kamerlin; Markus Meuwly; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Molecular Dynamics; Empirical Valence Bond; Enzyme Catalysis; Amyloid Beta; Aβ; Triosephosphate Isomerase; TIM; Computational Biochemistry; Computational Enzymology; Biokemi; Biochemistry;

    Sammanfattning : Proteins are a structurally diverse and functionally versatile class of biomolecules. They perform a variety of life-sustaining biological processes with utmost efficiency. A profound understanding of protein function requires knowledge of its structure. LÄS MER