Sökning: "patch clamp"

Visar resultat 1 - 5 av 88 avhandlingar innehållade orden patch clamp.

  1. 1. Patch clamp and calcium studies on human colonic mucosal cells

    Författare :Peter Sand; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Human; colon crypt; HT29; K channel; Ca oscillations; volume regulation; patch clamp;

    Sammanfattning : Background: The main function of the large intestine is to fine-tune electrolyte and water balance. This is achieved through the consorted action of ion channels, transporters and pumps. Failures of this system can lead to diarrhoea. LÄS MER

  3. 2. Methods for elucidating membrane protein structure and function

    Författare :Carolina Trkulja; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; patch clamp; antibodies; mass spectrometry; ion channels; proteomics; fluorescence; TRPV1; cholesterol.; limited proteolysis; Membrane proteins;

    Sammanfattning : Elucidation of the structure-function relationship of membrane proteins increases our understanding of many biological processes, both physiological and pathological. However, membrane proteins have been notoriously difficult to study, having part of their structure embedded into the lipid cell membrane. LÄS MER

  4. 3. Synaptic Integration of Hippocampal Neurons Generated in the Adult Brain: Influence of a Pathological Environment

    Författare :Katherine Jakubs; Lund Neurologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; neuropsykologi; neurofysiologi; Neurologi; synaptic plasticity; GABA; glutamate; GFP; Neurology; neuropsychology; neurophysiology; hippocampus; dentate gyrus; neurogenesis; LPS; inflammation; epilepsy; SE; patch-clamp;

    Sammanfattning : The adult brain constantly produces new neurons from endogenous neural progenitor cells located in at least two regions, the subgranular zone (SGZ) in dentate gyrus of the hippocampus, and the subventricular zone (SVZ). Major efforts are underway to discover the functional significance of new neurons in the adult brain. LÄS MER

  5. 4. Electrophysiology of pancreatic A-, B- and D-cells studied in intact mouse islets of Langerhans

    Författare :Sven Göpel; Institutionen för experimentell medicinsk vetenskap; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; somatostatin; glucagon; insulin; patch-clamp; membrane potential; Pancreatic islet; ion channel; diabetes; Physiology; Fysiologi;

    Sammanfattning : Abstract The electrophysiological properties of insulin-secreting b-cells, glucagon-releasing a-cells and somatostation-producing d-cells in intact mouse pancreatic islets were investigated using the perforated patch whole-cell technique. It is demonstrated that it is possible to functionally identify the different cell types in situ. LÄS MER

  7. 5. A Solution Exchange Platform for Exposure of Cells to Chemical Waves

    Författare :Jessica Olofsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; waveform synthesis; solution exchange; cell signalling; patch clamp; receptor; high through-put; microfluidics; microfabrication; channel; chemical oscillations; ion channel; poly dimethylsiloxane ; drug discovery;

    Sammanfattning : This thesis presents the development, characterization and suggested applications of a platform for controlling the chemical environment around a cell or a cell constituent. The platform is unique in its capability to combine rapid solution switching with precise timing and access to a manifold of solution environments. LÄS MER