Sökning: "Leif Isaksson"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden Leif Isaksson.

  1. 1. Studies on the functional interaction of translation initiation factor IF1 with ribosomal RNA

    Författare :Jaroslav Belotserkovsky; Leif Isaksson; Diarmaid Hughes; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; ribosome; rRNA mutation; molekylärgenetik; Molecular Genetics;

    Sammanfattning : Translation initiation factor IF1 is a small, essential and ubiquitous protein factor encoded by a single infA gene in bacteria. Although several important functions have been attributed to IF1, the precise reason for its indispensability is yet to be defined. LÄS MER

  3. 2. Study on the Function of Translation Initiation Factor IF1

    Författare :Victor Croitoru; Leif A. Isaksson; Kim Kusk Mortensen; Stockholms universitet; []
    Nyckelord :translation; initiation factor IF1; mutagenesis; A-site; RNA chaperone; NATURAL SCIENCES; NATURVETENSKAP;

    Sammanfattning : Initiation is the first step in protein biosynthesis representing a fundamental event in cell life which determines fidelity, efficiency and regulation of gene expression. In addition to the ribosome and mRNA, three protein factors IF1, IF2 and IF3 are involved in the initiation of translation in prokaryotes. LÄS MER

  4. 3. The Role of the Escherichia coli RNA Polymerase β´Jaw in Transcription

    Författare :Josefine Ederth; Leif A Isaksson; Anne Farewell; Stockholms universitet; []
    Nyckelord :mikrobiologi;

    Sammanfattning : Transcription, the central step in gene expression and regulation, is carried out by the DNA-dependent RNA polymerase (RNAP). Bacterial RNAP is a complex molecular machine in which the network of interacting parts and their movements, including contacts to nascent RNA and the DNA template, are at best partially understood. LÄS MER

  5. 4. Studies on translation initiation and gene expression in Escherichia coli

    Författare :Ernesto I. Gonzalez de Valdivia; Leif Isaksson; Stephen Douthwaite; Stockholms universitet; []
    Nyckelord :Downstream region; NGG codons; abortive translation; drop-off; Shine-Dalgarno sequence; gene expression and Escherichia coli; NATURAL SCIENCES; NATURVETENSKAP;

    Sammanfattning : In prokaryotes, several mRNA sequences surrounding the initiation codon have been found to influence the translation process; these include the downstream region and its codon context, the Shine-Dalgarno sequence and the S1 ribosomal protein-binding site. In this thesis, the purpose has been to study the role of the downstream region and Shine-Dalgarno-like sequences on early translation elongation and gene expression in Escherichia coli. LÄS MER

  7. 5. Studies on Translation Initiation and Termination in Escherichia coli

    Författare :Georgina Ibrahim Isak; Leif Isaksson; Suparna Sanyal; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; translation; ribosome; IF1; RF1; kasugamycin; tRNA; molekylärgenetik; Molecular Genetics;

    Sammanfattning : Translation initiation factor 1 (IF1) has been shown to be an RNA chaperone. In order to find functional interactions that IF1 may have with rRNA, we have isolated second-site suppressors of a cold-sensitive IF1 mutant. LÄS MER