Sökning: "Galactose dehydrogenase"

Hittade 5 avhandlingar innehållade orden Galactose dehydrogenase.

1. 1. Gene fusion in protein engineering : design of novel peptides and bifunctional enzymes

   Författare :Sarah Fredriksson; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cadmium; Bifunctional enzyme; Catalytic triad; Channeling; Coenzyme recycling; Galactose dehydrogenase; ß-Galactosidase; Gene fusion; Hexapeptide; Lactate dehydrogenase; Linker region; Multifunctional enzymes; Oligomeric protein; NADH; Phage; OmpA; Biochemistry; Biokemi; metabolism; Biotechnology; Bioteknik;

   Sammanfattning : The gene fusion technique is widely used in protein engineering and in this thesis it constitutes the basis of both the construction of artificial bifunctional enzymes and the display of two novel peptides. Artificial bifunctional enzymes have been used as model systems in the studies of native multifunctional enzymes and proximity effects within these complexes. LÄS MER

3. 2. Engineering Saccharomyces cerevisiae for mixed-sugar fermentation

   Författare :Rosa Garcia Sanchez; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; metabolic engineering; evolutionary engineering; promoter; mixed-sugar utilization; galactose; arabinose; xylose; Saccharomyces cerevisiae; fermentation; ethanol; phosphoglucomutase;

   Sammanfattning : Efficient fermentation of all the monomeric sugars derived from lignocellulose is crucial to increase the economy of bioethanol production, since they can account for a considerable fraction of the fermentable sugars in the raw material. This thesis describes the engineering of Saccharomyces cerevisiae strains for improved xylose, galactose and/or arabinose utilization. LÄS MER

4. 3. A kinetic study of metabolite transfer in coupled two-enzyme reactions

   Författare :Henrik Pettersson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; Free diffusion; Enzyme kinetics; Channelling; Dehydrogenase; Fusion protein; Biochemistry; Proximity effect; metabolism; Biokemi;

   Sammanfattning : There are two fundamentally different ways by which a metabolite can be transferred between enzymes in metabolic pathways. If the two sequential enzymes cannot form a complex with each other, the metabolite must first be released from the producing enzyme to the reaction medium and then be transported to the consuming enzyme, via the reaction medium, by free diffusion. LÄS MER

5. 4. Genetically Engineered Plants: Improved stress tolerance, growth and facilitated protein purification

   Författare :Gösta Lilius; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glycine betaine; gene; freezing; flooding; drought; dicoteledones; desiccation; compatible solutes; cold; choline dehydrogenase; chill; CDH; betaine; betA; alkaloids anoxia; affinity purification; affinity tags; handles; hemoglobin; hypoxia; low temperatu; Biotechnology; Bioteknik;

   Sammanfattning : Heterologous genes were expressed in plants in which the overall goal was to increase the value of the final crop. Various aspects were explored and evaluated, such as improving the stress tolerance, enhancing the availability of oxygen in growing cells and producing rare proteins with increased degrees of purity. LÄS MER

7. 5. Metabolic Engineering of Xylose-Utilising Saccharomyces cerevisiae Strains. A Closer Look at Recombinant Strains Based on the Xylose Reductase-Xylitol Dehydrogenase Pathway

   Författare :Marie Jeppsson; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi; microarrays; cofactors; XK; XDH; XR; lignocellulose; xylose; ethanol; Saccharomyces cerevisiae; yeast;

   Sammanfattning : Saccharomyces cerevisiae produces ethanol efficiently from the hexose sugars in lignocellulose hydrolysates, but it can not utilise pentose sugars such as xylose and arabinose. Stable xylose-utilising S. LÄS MER