Sökning: "xylose"

Visar resultat 1 - 5 av 99 avhandlingar innehållade ordet xylose.

  1. 1. The GRE3 encoded aldo-keto reductase and its influence on xylose fermentation in recombinant Saccharomyces cerevisiae strains

    Författare :Karin Träff; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biochemical technology; Biokemisk teknik; dihydroxyacetone phosphate; xylose reductase; xylose; xylose isomerase; GRE3; Saccharomyces cerevisiae; aldo-keto reductase;

    Sammanfattning : The aim of the work presented in this thesis was to improve ethanol formation from xylose in recombinant Saccharomyces cerevisiae strains. This was achieved by decreasing the xylitol by-product formation. Gre3p is the major xylose-reducing enzyme in S. cerevisiae. LÄS MER

  3. 2. Improving initial xylose metabolism in recombinant Saccharomyces cerevisiae

    Författare :Márk Gárdonyi; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biokemisk teknik; Biochemical technology; xylose isomerase; xylose transport; xylose fermentation; Saccharomyces cerevisiae; metabolic engineering;

    Sammanfattning : The aim of the thesis is to improve the initial steps of xylose metabolism in recombinant Saccharomyces cerevisiae. S. cerevisiae takes up xylose of poor affinity by means of hexose transporters. Metabolic control analysis was used to investigate whether the low xylose utilisation rate is due to inefficient transport. LÄS MER

  4. 3. Genetic Traits Beneficial for Xylose Utilization by Recombinant Saccharomyces cerevisiae

    Författare :Oskar Bengtsson; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Saccharomyces cerevisiae; metabolic engineering; fermentation; xylose; ethanol; promoter; xylose reductase; cofactor; validation; transcriptome;

    Sammanfattning : Saccharomyces cerevisiae ferments hexoses in lignocellulosic hydrolysates under anaerobic conditions with high rates and ethanol yields. However, S. cerevisiae is naturally unable to utilize the pentose fraction of the hydrolysates. Xylose is the most abundant pentose sugar, and although recombinant S. LÄS MER

  5. 4. Redox balancing in recombinant strains of Saccharomyces cerevisiae

    Författare :Mikael Anderlund; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; xylose isomerase; xylitol dehydrogenase; xylose reductase; glycerol; xylitol; xylose; E. coli; A. vinelandii; T. thermophilus; S. cerevisiae; P. stipitis; transhydrogenase.; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : In metabolically engineered Saccharomyces cerevisiae expressing Pichia stipitis XYL1 and XYL2 genes, encoding xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively, xylitol is excreted as the major product during anaerobic xylose fermentation and only low yields of ethanol are produced. This has been interpreted as a result of the dual cofactor dependence of XR and the exclusive use of NAD+ by XDH. LÄS MER

  7. 5. Metabolic Control Points in Ethanolic Fermentation of Xylose by Saccharomyces cerevisiae

    Författare :David Runquist; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Transcriptomics; Xylose reductase; Saccharomyces cerevisiae; Xylose; Ethanol; Transport; Lignocellulose;

    Sammanfattning : Baker’s yeast, Saccharomyces cerevisae, has been engineered to utilize the pentose sugar xylose present in lignocellulose biomass for the production of ethanol. Currently ethanol production from xylose is slow which limits the implementation of said strains in industrial production. LÄS MER