Sökning: "Fluorometry"

Visar resultat 1 - 5 av 9 avhandlingar innehållade ordet Fluorometry.

  1. 1. Physiological and clinical studies of calcium-regulating hormones calcitonin and parathyroid hormone-related protein

    Författare :Haiqin Rong; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :bone metabolism; breast cancer; calcitonin; calcium; exercise; lanthanide; milk; neonate; osteoporosis; parathyroid hormone-related protein; parturition; polymerase chain reaction; time-resolved fluorometry;

    Sammanfattning : The present studies were performed to elucidate several physiological and clinical questions of calcitonin (CT) and parathyroid hormone-related protein (PTHrP) such as: a) Does age influence the basal and calcium-stimulated levels and circulating molecular forms of CT in healthy females? b) Are osteoporotic patients lacking circulating monomeric CT? c) How is salmon CT (sCT) absorbed and cleared during treatment of diseases? d) Is there any effect of acute physical activity on circulating CT, PTH, PTHrP and bone metabolic markers? e) Is there an absorbance of PTHrP in milk by the neonate? and f) Are there any interactions between CT and PTHrP? In addition, by use of time-resolved dissociation and enhancement lanthanide fluorometry, the effort was put to develop two-site immunofluorometric assays (IFMA) for sCT and PTHrP as well as a quantitative competitive PCR (QC-PCR) for measuring PTHrP mRNA and use these methods in some of the investigations. Heterogeneity of CT forms in serum was disclosed after immunoextraction, fast protein liquid chromatography (FPLC) and radioimmunoassay (RIA) in healthy females and osteoporotic patients. LÄS MER

  3. 2. Calcium signaling in astroglial cells. Noradrenergic effects and regulation

    Författare :Håkan Muyderman; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Astrocytes; Neurons; Noradrenaline; Glutamate; Fluorometry; Fura-2; Fluo-3; PBFI; Digital imaging; Voltage-patch-clamp; Whole-cell currents; Calcium; Potassium; Intercellular communication; Glutamate release; Neuron-glial interactions.;

    Sammanfattning : Astroglial cells are the most common cell type in the central nervous system and express a wide array of ion channels and neurotransmitter receptors. The cells organize into multicellular networks and exhibit a calcium-based excitability in which receptor-mediated calcium elevations propagate as intercellular waves over considerable distances. LÄS MER

  4. 3. Cytofluorometry : technique and applications

    Författare :Ingemar Rundquist; Linköpings universitet; []
    Nyckelord :MEDICINE; MEDICIN;

    Sammanfattning : The aims of this investigation were to examine the conditions for rapid fluorescence measurements on cellular material, to improve the performance of measurement systems for microscope fluorometry, and to apply this technique in studies on mast cell biology. Fluorescence fading, a general complication of cytofluorometry, was studied during illumination times in the millisecond range, and a new fading phenomenon characterized by short duration and rapid recovery was described. LÄS MER

  5. 4. Metabolic analysis of shikimic acid producing Escherichia coli

    Författare :Louise Johansson; Avdelningen för kemiteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Kemiteknik och kemisk teknologi; Biokemi; metabolism; Chemical technology and engineering; transcriptome analysis; phosphorus-limitation; carbon-limitation; fed-batch; chemostat; batch; fermentation technology; Escherichia coli; shikimic acid; Biotechnology; Bioteknik; Metabolism; Biochemistry; principal component analysis; 2D-fluorescence spectrophotometry; metabolic flux analysis; carbon-rich;

    Sammanfattning : Shikimic acid production using recombinant E.coli has been developed during the past 15 years due to the increased demand of this product, primarily as a starting material for the anti-influensa medicine Tamiflu?. A detailed metabolic analysis of a shikimic acid producing E. LÄS MER

  7. 5. Phosphorylation in State Transition : Less cause more effect

    Författare :Jakob Damkjaer; Jansson Stefan; Eva-Mari Aro; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Photosynthesis; Photoacclimation; State Transtion; LHCII Phosphorylation; Lhcb3; Lhcb6; Plant physiology; Växtfysiologi; molekylärbiologi; Molecular Biology;

    Sammanfattning : Study of the Arabidopsis thaliana knockout mutant lacking Lhcb3 (koLhcb3) have revealed a close similarity to the wild type plants. Growth rate, NPQ, qP, Φ(PSII), circular dichroism spectra, pigment composition and content of LCHII trimers have been found to be unaffected by this mutation. LÄS MER