Sökning: "spin-labels"
Visar resultat 1 - 5 av 9 avhandlingar innehållade ordet spin-labels.
1. Theoretical studies of EPR parameters of spin-labels incomplex environments
Sammanfattning : This thesis encloses quantum chemical calculations performed in the framework of density functional response theory for evaluating electron paramagnetic resonance (EPR) spin Hamiltonian parameters of various spin-labels in different environments. These parameters are the well known electronic g-tensor and the nitrogen hyperfine coupling constants, which are extensively explored in this work for various systems. LÄS MER
2. Lipase-Lipid Interactions Studied by Site-Directed Labeling and Biological Spectroscopy
Sammanfattning : This thesis describes the study of lipase–lipid interactions of the triglyceride lipase Thermomyces lanuginosus lipase, TLL, by use of site-directed chemical labeling and biological spectroscopy. Several single-cysteine variants of TLL were produced, and labeled with probes containing either an affinity-, a fluorescence- or a spin-label functionality. LÄS MER
3. Structure Analysis of a Transient Protein Complex - NMR, X-ray crystallographic and Mass Spectrometric Studies of the Soluble Domains of the Proton-Pump Transhydrogenase from E. coli
Sammanfattning : Interactions between macromolecules in the cell are fundamental to life. However, many interactions are of a transient nature and are as such difficult to study at the atomic level using existing techniques. LÄS MER
4. Molecular dynamics in membrane systems : an ESR spin label study
Sammanfattning : The ESR spin labeling technique has been used to investigate order and motion in medel lipid systems and in biological membranes under various experimental conditions.The effects of CaCl2, MgCl2, LaCl3 and some alkali halides on B. LÄS MER
5. Studies of Local Interactions between and within Proteins using Site-Directed Labeling Techniques
Sammanfattning : Proteins are essential participants in virtually all cellular processes. The key to the understanding of the function of a certain protein is a detailed knowledge about its atomic three-dimensional structure. LÄS MER