Sökning: "semi-rational enzyme engineering"
Visar resultat 1 - 5 av 6 avhandlingar innehållade orden semi-rational enzyme engineering.
1. Protein Engineering of Candida antarctica Lipase A : Enhancing Enzyme Properties by Evolutionary and Semi-Rational Methods
Sammanfattning : Enzymes are gaining increasing importance as catalysts for selective transformations in organic synthetic chemistry. The engineering and design of enzymes is a developing, growing research field that is employed in biocatalysis. LÄS MER
2. Multidimensional engineering for the production of fatty acid derivatives in Saccharomyces cerevisiae
Sammanfattning : Saccharomyces cerevisiae , also known as budding yeast, has been important for human society since ancient time due to its use during bread making and beer brewing, but it has also made important contribution to scientific studies as model eukaryote. The ease of genetic modification and the robustness and tolerance towards harsh conditions have established yeast as one of the most popular chassis in industrial-scale production of various compounds. LÄS MER
3. Mutational Analysis and Redesign of Alpha-class Glutathione Transferases for Enhanced Azathioprine Activity
Sammanfattning : Glutathione transferase (GST) A2-2 is the human enzyme most efficient in catalyzing azathioprine activation. Structure-function relationships were sought explaining the higher catalytic efficiency compared to other alpha class GSTs. LÄS MER
4. Directed Evolution of Glutathione Transferases with Altered Substrate Selectivity Profiles : A Laboratory Evolution Study Shedding Light on the Multidimensional Nature of Epistasis
Sammanfattning : Directed evolution is generally regarded as a useful approach in protein engineering. By subjecting members of a mutant library to the power of Darwinian evolution, desired protein properties are obtained. Numerous reports have appeared in the literature showing the success of tailoring proteins for various applications by this method. LÄS MER
5. In silico protein design for the enhancement of protein stability and function
Sammanfattning : Enzymes are natures catalysts that increase the rate of a chemical reaction. The increased rate of a reaction is required to be able to sustain life. Despite the huge impact of enzymes, they are not perfect catalysts. LÄS MER