Sökning: "protein targets"

Visar resultat 16 - 20 av 779 avhandlingar innehållade orden protein targets.

  1. 16. The p97 ATPase and the Drosophila Proteasome : Protein Unfolding and Regulation

    Författare :Kristian Björk Grimberg; Patrick Young; Neus Visa; Lars Thelander; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; proteasome; ubiquitin; p97; unfolding; regulation; RNAi screen; high-throughput screen; cnc; basic leucine zipper; transcription factors; proteasome inhibition; Molecular biology; Molekylärbiologi; Molecular Biology; molekylärbiologi;

    Sammanfattning : For all living systems, there is a requirement to recycle and regulate proteins. In eukaryotic organisms this is accomplished by the proteasome. The p97 ATPase is another highly conserved and essential complex present throughout the eukaryotic cell. LÄS MER

  3. 17. Epitope mapping of antibodies towards human protein targets

    Författare :Barbara Hjelm; Mathias Uhlén; Larry Gold; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; antibody; antibody validation; biomarker; epitope mapping; peptide array; proteomics; RBM3; staphylococcal surface display;

    Sammanfattning : This thesis, based on five research papers, presents results from development and evaluation ofmethods for identifying the interaction site of antibodies on their antigens and the functional investigation of these in different assays. As antibodies have proven to be invaluable tools in diagnostics, therapy and basic research, the demand of characterizing these binding molecules has increased. LÄS MER

  4. 18. Diving into short linear motifs : Large-scale identification of endogenous and host-pathogen protein-protein interactions and further characterized by deep mutational scanning

    Författare :Caroline Benz; Ylva Ivarsson; Birthe B. Kragelund; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein-protein interactions; short linear motif; endogenous interactions; host-pathogen; viral hijacking; deep mutational scanning; Biokemi; Biochemistry;

    Sammanfattning : Short linear motifs (SLiMs) are protein-protein interaction sites that play an essential role in distinct cellular processes. Those interactions are challenging to capture by common high-throughput methods. LÄS MER

  5. 19. Exploring the interactome of EF-hand proteins

    Författare :Mikael Bauer; Biofysikalisk kemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; EF-hand; calcium; STIM; stromal interaction molecule; secretagogin; Calmodulin; calbindin D28k; protein-protein interactions; zinc; protein array;

    Sammanfattning : EF-hand proteins are central in many cellular processes being the main group of Ca2+-sensing and buffering proteins in the cell. In the work presented in this thesis we study ligand interactions of three EF-hand proteins; calmodulin, calbindin D28k and secretagogin. LÄS MER

  7. 20. Purine and Pyrazolopyrimidine Derivatives: Design and Synthesis of Chemical Tools for Biological Applications

    Författare :David Bliman; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; purine; pyrazolopyrimidine; caged compounds; protein kinases; protein-protein interactions; fluorescence; organic synthesis;

    Sammanfattning : Purines can be found in a multitude of naturally occurring compounds with a range of functions. This thesis describes the design and synthesis of purines and structurally related pyrazolopyrimidine derivatives intended for biological applications. LÄS MER