Sökning: "RNAi screen"

Visar resultat 1 - 5 av 15 avhandlingar innehållade orden RNAi screen.

  1. 1. Connecting Systemic RNAi to the Endomembrane System in Caenorhabditis elegans

    Författare :Benjamin T. Holmgren; Andrea Hinas; Gerhart Wagner; Peter Brodersen; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Systemic RNAi; RNAi inheritance; Endomembrane system; Late endosome; SID-5; SEC-22; SYX-6; C12D8.1; RNA; Biology with specialization in Molecular Cell Biology; Biologi med inriktning mot molekylär cellbiologi;

    Sammanfattning : RNA interference (RNAi) is a gene regulation mechanism conserved among eukaryotes. To silence gene expression, RNAi relies on a short single-stranded guide RNA to steer the RNA-induced Silencing Complex (RISC) to mRNAs with guide strand-complementary sequences. RNAi is a highly membrane-associated process. LÄS MER

  3. 2. Systemic RNAi Relies on the Endomembrane System in Caenorhabditis elegans

    Författare :Yani Zhao; Andrea Hinas; Staffan Svärd; Simon Tuck; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Systemic RNAi; RNAi inheritance; late endosomes MVBs; SID-5; SEC-22; SYX-6; C12D8.1; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Sammanfattning : The membrane system of a eukaryotic cell is a large and complex system handling the transport, exchange and degradation of many kinds of material. Recent research shows that double-stranded RNA (dsRNA) mediated gene silencing (RNA interference) is a membrane related process. LÄS MER

  4. 3. The p97 ATPase and the Drosophila Proteasome : Protein Unfolding and Regulation

    Författare :Kristian Björk Grimberg; Patrick Young; Neus Visa; Lars Thelander; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; proteasome; ubiquitin; p97; unfolding; regulation; RNAi screen; high-throughput screen; cnc; basic leucine zipper; transcription factors; proteasome inhibition; Molecular biology; Molekylärbiologi; Molecular Biology; molekylärbiologi;

    Sammanfattning : For all living systems, there is a requirement to recycle and regulate proteins. In eukaryotic organisms this is accomplished by the proteasome. The p97 ATPase is another highly conserved and essential complex present throughout the eukaryotic cell. LÄS MER

  5. 4. Identification of intrinsic regulators in normal and malignant human hematopoiesis. An RNA-interference approach

    Författare :Roman Galeev; Avdelningen för molekylärmedicin och genterapi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; hematopoietic stem cell; RNA interference; Acute myeloid leukemia; myelodysplastic syndrome; genetic screens; next generation sequencing; genetic regulators;

    Sammanfattning : Bildandet av blodceller, hematopoesen, utgår från ett litet antal av de så kallade hematopoetiska stamceller som sitter i vår benmärg. Dessa celler har förmågan att ge upphov till alla mogna blodcellstyper av de tre hematopoetiska linjerna, samtidigt som de genom celldelning bibehåller sin egna mängd. LÄS MER

  7. 5. Signaling pathways in Drosophila immunity

    Författare :Shiva Seyedoleslami Esfahani; Ylva Engström; Steven Wasserman; Stockholms universitet; []
    Nyckelord :Drosophila immunity; signaling pathways; Cactus; NFκB; Molecular Biology; molekylärbiologi;

    Sammanfattning : Drosophila relies on innate immunity to protect itself from its hostile environment throughout its life cycle. Despite the remarkable progress in understanding many aspects of Drosophila immunity, there are still big gaps in our knowledge. LÄS MER