Sökning: "plasmid maintenance"

Hittade 5 avhandlingar innehållade orden plasmid maintenance.

  1. 1. Novel approaches to mucosal vaccine development Strategies in vaccine antigen production, construction of a novel mucosal adjuvant and studies of its mode of action

    Författare :Manuela Terrinoni; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; vaccine development; plasmid maintenance; Gram-negative bacteria; ; essential genes; complementation; Cholera Toxin; adjuvanticity; NF-kB; mmCT; Gram-negative bacteria;

    Sammanfattning : Although most infections begin at a mucosal surface and may be prevented by effective vaccine stimulation of the local mucosal immune system, there are so far only a few mucosal vaccines available for human use. This thesis spans several areas that are important for future development of mucosal vaccines. LÄS MER

  3. 2. Global regulatory factors that impact metabolic and lifestyle choices in Pseudomonas putida

    Författare :Lisa Wirebrand; Victoria Shingler; Matthew Francis; Carlos Balsalobre; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Pseudomonas putida; c-di-GMP; motility; ppGpp; DksA; biofilm dispersal; transcriptional and translational regulation; dmp-system; phenol catabolism; carbon repression control; plasmid stability;

    Sammanfattning : Pseudomonas putida strains have a broad metabolic capacity and are innately resistant to many harmful substances – properties that make them of interest for a number of industrial and biotechnological application. They can rapidly adapt to changes in physico-chemical parameters in the soil and water environments they naturally inhabit. LÄS MER

  4. 3. Studies of Intracellular Transport and Anticancer Drug Action by Functional Genomics in Yeast

    Författare :Marie Gustavsson; Hans Ronne; Anders Blomberg; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; 5-fluorouracil; tRNA modifications; Saccharomyces cerevisiae; carbamoyl phosphate synthase; V-ATPase; Functional genomics; Funktionsgenomik;

    Sammanfattning : This thesis describes the use of functional genomics screens in yeast to study anticancer drug action and intracellular transport. The yeast Saccharomyces cerevisiae provides a particularly useful model system for global drug screens, due to the availability of knockout mutants for all yeast genes. LÄS MER

  5. 4. Mitochondrial plasmids, genetic conflicts and sex-determination in Silene vulgaris

    Författare :Helene Andersson; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Silene vulgaris; sex-determination; mitochondrial plasmids; genetic conflicts; Botanik; Botany;

    Sammanfattning : Plants like all other eukaryotic organisms carry most of their DNA in the nucleus. In addition, plants also have both chloroplasts and mitochondria that contain their own genetic material. The genomes in chloroplasts and mitochondria control certain vital processes, but most often the organelles act under strict nuclear control. LÄS MER

  7. 5. Physiological Engineering of Xylose Utilisation by Recombinant Saccharomyces cerevisiae

    Författare :Nina Quednau Meinander; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein burden; physiology; fed-batch fermentation; chemostat; recombinant Saccharomyces cerevisiae; ethanol; xylose; xylitol; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : Xylitol production by recombinant, XYL1-expressing Saccharomyces cerevisiae was investigated in fed-batch fermentation using different cosubstrates for growth, and generation of reduced cofactors and maintenance energy. Xylose was converted into xylitol with 1:1 yield. LÄS MER