Sökning: "pcr lund"

Visar resultat 1 - 5 av 46 avhandlingar innehållade orden pcr lund.

1. 1. Novel planar and particle-based microarrays for point-of-care diagnostics

   Författare :Gustav Svedberg; Helene Svahn Andersson; Jesper Gantelius; Fridtjof Lund-Johansen; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Planar microarrays; Particle-based microarrays; Point-of-care diagnostics; Colorimetric detection; Signal enhancement; Isothermal DNA amplification; Solid-phase DNA amplification; Biotechnology; Bioteknologi;

   Sammanfattning : Point-of-care assays are easy-to-use, portable and inexpensive tests that canbe used to aid diagnostics by measuring levels of disease-specific moleculesin settings where access to advanced laboratory equipment and trainedpersonnel are limited, such as at the patient's bedside or in low resourceparts of developing countries. In order to achieve high multiplexingcapacities, such assays can be based on planar microarrays consisting ofspots immobilized on a flat surface or on particle-based microarrays basedon populations of encoded particles. LÄS MER

3. 2. LYME BORRELIOSIS Detection and identification of the infecting genotype

   Författare :Katharina Ornstein; Sektion III; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Parasitologi människa och djur ; Parasitology human and animal ; Infektioner; Infections; PCR; genospecies; Lyme borreliosis; Borrelia burgdorferi; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

   Sammanfattning : Lyme borreliosis is caused by the spirochete Borrelia burgdorferi sensu lato, a bacterium with a parasitic life-style, which is transmitted between hosts by the Ixodes tick-vector. Humans will incidentally become infected. The resulting illness can involve the skin, nervous system, joints and heart. LÄS MER

4. 3. Mammographic density. A marker of treatment outcome in breast cancer?

   Författare :Ida Dalene Skarping; Bröstcancer - prevention & intervention; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mammographic density; pCR; Neoadjuvant chemotherapy; Diagnostic precision;

   Sammanfattning : .... LÄS MER

5. 4. Real-time PCR for diagnosis of botulism and quantification of neurotoxin gene expression in Clostridium botulinum

   Författare :Ingrid Artin; Avdelningen för medicinsk mikrobiologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; detection; qRT-PCR; Clostridium botulinum; molecular methods;

   Sammanfattning : The objectives of the work presented in this thesis have been to develop molecular methods for (i) detection of Clostridium botulinum in food and clinical samples, and (ii) monitoring the expression of the neurotoxin gene, cnt, during different growth phases and under different growth conditions. For the diagnosis of botulism, a real-time PCR-based method including an internal amplification control for C. LÄS MER

7. 5. Neuropeptide Y - aspects of vasomotorresponses and receptor characterization

   Författare :Torun Nilsson-Bergdahl; Lund Medicin; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; vasoconstriction; RT-PCR; receptor characterization; potentiation; NPY Y1 receptor; NPY; mitogenesis; hypertension; human arteries; forearm blood flow; cell culture; antisense oligodeoxynucleotides; BIBP3226; vasodilatation.; Physiology; Fysiologi; Endocrinology; secreting systems; diabetology; Endokrinologi; sekretion; diabetologi;

   Sammanfattning : Abstract The aims of the present thesis were to examine neuropeptide Y (NPY) responses in different human and guinea pig vascular beds and in cultured human vascular smooth muscle cells (VSMC) and to characterize, with different NPY agonists and the specific NPY Y1 receptor antagonist (BIBP3226), the NPY receptors involved. NPY-induced strong and potent contractions in human cerebral and subcutaneous arteries and in guinea pig basilar arteries. LÄS MER