Sökning: "TK6 cells"

Hittade 5 avhandlingar innehållade orden TK6 cells.

  1. 1. Evolving the Methodology for Detection of Primary DNA Damage : Development, adaptation and assessment of the single cell gel electrophoresis (comet) assay

    Författare :Erik Bivehed; Björn Hellman; Hanna Karlsson; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Comet assay; DNA damage; DNA strand breaks; Flash-comet; Genotoxicity; In vitro; Single cell gel electrophoresis; Polymerase assisted DNA damage assay PADDA ; TK6 cells; UCDAS; U937 cells; Toxikologi; Toxicology; Farmaceutisk vetenskap; Pharmaceutical Science;

    Sammanfattning : Deoxyribonucleic acid (DNA) is one of the most important molecules in nature. It is the fundamental carrier of evolutionary information and constitutes the genetic blueprint of all living organisms. Being the sole source of information, it is vital for the cell to transmit the correct genetic information from generation to generation. LÄS MER

  3. 2. The role of MTH1 in ultraviolet radiation-induced mutagenesis

    Författare :Asal Fotouhi; Siamak Haghdoost; Harry Vrieling; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; ultraviolet radiation; MTH1; reactive oxygen species; 8-oxo-dGTP; 2-OH-dATP; micronucleus; molekylärgenetik; Molecular Genetics;

    Sammanfattning : Ultraviolet radiation (UVR) is known to be highly mutagenic. What types of DNA lesions that are induced by different UVR wavelengths are still a matter of debate. UVR induces mutagenesis mostly by the formation of photoproducts and the induction of reactive oxygen species (ROS). LÄS MER

  4. 3. Role of MTH1 and MYH proteins in genotoxic effects of radiation

    Författare :Sara Shakeri Manesh; Siamak Haghdoost; William F. Morgan; Stockholms universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MTH1; MYH; gamma radiation; UV radiation; oxidative stress; low dose rate ionizing radiation; molekylärgenetik; Molecular Genetics;

    Sammanfattning : Humans are constantly exposed to different types of radiations. It has been suggested that low dose and low dose rate of γ-radiation as well as ultra violet A (UVA) induce oxidative stress in cells that may promote mutations. LÄS MER

  5. 4. In vitro and in vivo aspects of intrinsic radiosensitivity

    Författare :Karl Brehwens; Andrzej Wojcik; Siamak Haghdoost; Wolfgang Dörr; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Radiation biology; changing dose rates; cytogenetics; hypothermia; X-rays; radiotherapy; osteoradionecrosis; biomarkers; oxidative stress; individual radiosensitivity; molekylär biovetenskap; Molecular Biosciences;

    Sammanfattning : This thesis focuses on how physical and biological factors influence the outcome of exposures to γ/X-rays. That the dose rate changes during real life exposure scenarios is well-known, but radiobiological data from exposures performed at increasing or decreasing dose rates is lacking. LÄS MER

  7. 5. Developing methods for mapping genetic heterogeneity in tumors : from bulk to single-cell resolution

    Författare :Michele Simonetti; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Tumors are complex ecosystems composed of billions of cells that constantly evolve over time. Intra-tumor heterogeneity (ITH) represents the diversity of this complex environment quantifiable both at the genetic and phenotypic level. Recently, next-generation sequencing (NGS) costs have dropped and NGS has become the standard tool for studying ITH. LÄS MER