Sökning: "citrate synthase"

Visar resultat 1 - 5 av 22 avhandlingar innehållade orden citrate synthase.

1. 1. Mitochondrial function in human skeletal muscle: with special reference to exercise and training

   Författare :Michail Tonkonogi; Högskolan Dalarna; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :aerobic metabolism; oxidative phosphorylation; mitochondria; oxygen utilisation; citrate synthase; ADP sensitivity; ATP; muscle fibres; hydrogen ions; lactate; free radicals; proton leak; uncoupling; exercise; aerobic metabolism; oxidative phosphorylation; mitochondria; oxygen utilisation; citrate synthase; ADP sensitivity; ATP; muscle fibres; hydrogen ions; lactate; free radicals; proton leak; uncoupling; exercise;

   Sammanfattning : The overall objective of this thesis was to study the adaptation of oxidative function in human skeletal muscle to acute exercise of different modes, intensities and durations, and to endurance training. The effects of endurance training on mitochondrial function were evaluated in cross-sectional and longitudinal studies by measurements of mitochondrial oxygen consumption in isolated mitochondria and permeabilised muscle fibres and measurements of mitochondrial ATP production rate in isolated mitochondria. LÄS MER

3. 2. Engineering cytosolic acetyl-CoA metabolism in Saccharomyces cerevisiae

   Författare :Yiming Zhang; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; reverse engineering; flavodoxin; genomic DNA sequencing; ferredoxin; histone deacetylase; adaptive evolution; ferredoxin flavodoxin NADP reductase; pyruvate decarboxylase; citrate synthase; hexose transporter; yeast; mitochondria; aerobic growth; metabolic engineering.; acetyl-CoA; central carbon metabolism;

   Sammanfattning : A Saccharomyces cerevisiae strain carrying deletions in all three pyruvate decarboxylase genes (also called Pdc negative yeast) represents a non-ethanol producing platform strain for biochemical production. However, it cannot grow on glucose as the sole carbon source due to the lack of cytosolic acetyl-CoA for lipid biosynthesis. LÄS MER

4. 3. Molecular Simulation of Enzyme Catalysis and Inhibition

   Författare :Sinisa Bjelic; Johan Åqvist; Adrian J. Mulholland; Uppsala universitet; []
   Nyckelord :Theoretical chemistry; enzyme catalysis; enzyme inhibition; computer simulations; molecular dynamics; empirical valence bond method; structure-based inhibitor design; Teoretisk kemi;

   Sammanfattning : The reaction mechanisms for the hemoglobin degrading enzymes in the Plasmodium falciparum malaria parasite, plasmepsin II (Plm II) and histo-aspartic protease (HAP), have been analyzed by molecular simulations. The reaction free energy profiles, calculated by the empirical valence bond (EVB) method in combination with molecular dynamics (MD) and free energy perturbation (FEP) simulations are in good agreement with experimental data. LÄS MER

5. 4. Enantioselective synthesis of citric acid analogues

   Författare :Olof Dahlman; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : The enantioselective synthesis of (1S,2S)- and (1R,2S)- fluorocitric acid is described. Toxic fluorocitric acid formed enzymaticly from fluoroacetic acid has the 1R,2R configuration. The enzyme citrate (si)-synthase catalyzes the formation of all four isomers of fluorocitric acid. LÄS MER

7. 5. Rickettsia helvetica; Detection in arthropods and human tissues and its relation to clinical disease

   Författare :Kenneth Nilsson; Björn Olsen; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Communicable diseases; rickettsia; spotted fever; tick; PCR; myocarditis; sarcoidois; Infektionssjukdomar; Infectious diseases; Infektionssjukdomar; Infektionssjukdomar; Infectious Diseases;

   Sammanfattning : A relation between R. helvetica and perimyocarditis was, in two young men who died of sudden cardiac death during exercise in 1997, suggested by a seminested PCR, for three different genes, sequencing of the amplified products, documentation of a seroresponse and histopathologic changes in accordance with rickettsioses. LÄS MER